Cyclin T2 Protein (CCNT2) (AA 1-723) (Strep Tag)

Catalog No. ABIN3135945

Product Details

Target: Cyclin T2 (CCNT2)

Protein Type: Recombinant

Protein Characteristics: AA 1-723

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This Cyclin T2 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MASGRGASSR WFFTREQLEN TPSRRCGVEA DEELSHRQQA ANLIQDMGQR LNVSQLTINT AIVYMHRFYM HHSFTKFNRN IISPTALFLA AKVEEQARKL EHVIKVAHAC LHPLEPLLDT KCDAYLQQTQ ELVLLETIML QTLGFEITIE HPHTDVVKCT QLVRASKDLA QTSYFMATNS LHLTTFCLQY KPTVIACVCI HLACKWSNWE IPVSTDGKHW WEYVDPTVTL ELLDELTHEF LQILEKTPSR LKRIRNWRAM AKKPKVDGQV SETPLLGSSL VQNSILVDSV TGVPANPSFQ KPSTSTFPAP IPLNSGSTSV QDSRASDNLS VLAAGMPSTS YSLSSHQEWP QHPDSARTDP VYTQKQEATL SGSQYISFQQ GPSMALHSGL HHRPDKVADH SSAKQEYTHK AGSSKHHGPI PATPGMLPQK MSLDKYREKR KLETLDVDTR DHYLAAHAEQ QHKHGPAQAV TGTSVTSPIK MKLPLTNSDR PEKHVAEKKE RSGSLKLRIP IPPPDKGPSK EELKMKIKVA SSERHSSSDE GSGKSKHSSP HISRDHKEKH KEHPANRHHS SHKYLHMHSG GSKHTADGMP PTVLRSPVGL GPEGVSSASS ARKKLHSSEA SHNHHSKMSK SSKSAGSSSS SSSVKQYLSS HSSVFNHPLP PPPPVTYQVG YGHLSTLVKL DKKPVEPHGP EANHEYSTSS QHMDYKDTFD MLDSLLSAQG MNM
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Cyclin T2 (CCNT2)

Alternative Name: Ccnt2 (CCNT2 Products)

Synonyms: fb16c03 Protein, wu:fb16c03 Protein, wu:fj68b10 Protein, zgc:55690 Protein, ccnt2 Protein, cycT Protein, wu:fi17h07 Protein, zgc:55357 Protein, MGC81210 Protein, CCNT2 Protein, CYCT2 Protein, 2900041I18Rik Protein, C81304 Protein, CycT2 Protein, CycT2b Protein, cyclin T2 Protein, cyclin T2b Protein, cyclin T2a Protein, cyclin T2 S homeolog Protein, CCNT2 Protein, ccnt2b Protein, ccnt2a Protein, ccnt2.S Protein, ccnt2 Protein, Ccnt2 Protein

Background: Cyclin-T2 (CycT2),FUNCTION: Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II). The activity of this complex is regulated by binding with 7SK snRNA (By similarity). Plays a role during muscle differentiation, P-TEFB complex interacts with MYOD1, this tripartite complex promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation and binds the chromatin of promoters and enhancers of muscle-specific genes, this event correlates with hyperphosphorylation of the CTD domain of RNA pol II (PubMed:16245309, PubMed:23060074, PubMed:12037670). In addition, enhances MYOD1-dependent transcription through interaction with PKN1 (By similarity). Involved in early embryo development (PubMed:19364821). {ECO:0000250|UniProtKB:O60583, ECO:0000269|PubMed:12037670, ECO:0000269|PubMed:16245309, ECO:0000269|PubMed:19364821, ECO:0000269|PubMed:23060074}.

Molecular Weight: 80.3 kDa

UniProt: Q7TQK0