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Rubicon Protein (AA 1-956) (Strep Tag)

Crystallography grade KIAA0226 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Catalog No. ABIN3136015
  • Target See all Rubicon (KIAA0226) Proteins
    Rubicon (KIAA0226) (KIAA0226)
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-956
    Origin
    Mouse
    Source
    • 1
    • 1
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    Purification tag / Conjugate
    This Rubicon protein is labelled with Strep Tag.
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS)
    Sequence
    MRPEGAGMDL GGGDGERLLE KSRREHWQLL GNLKTTVEGL VSANCPNVWS KYGGLERLCR DMQNILYHGL IHDQVCCRQA DYWQFVKDIR WLSPHSALHV EKFISLHESD QSDTDSVSER AVAELWLQHS LQCHCLSAQL RPLLGDRQYI RKFYTETAFL LSDAHVTAML QCLEAVEQNN PRLLAQIDAS MFARKQESPL LVTKSQSLTA LPGSTYTPPA SYAQHSYFGS SSSLQSMPQS SHSSERRSTS FSLSGPSWQP QEDRECLSPA ETQTTPAPLP SDSTLAQDSP LTAQEMSDST LTSPLEASWV SSQNDSPSDV SEGPEYLAIG NPAPHGRTAS CESHSSNGES SSSHLFSSSS SQKLESAASS LGDQEEGRQS QAGSVLRRSS FSEGQTAPVA SGTKKSHIRS HSDTNIASRG AAGGPRNITI IVEDPIAEGG QYLCSGEGMF RRPSEGQSLI SYLSEQDFGS CADLEKENAH FSISESLIAA IELMKCNMMS QCLEEEEVEE EDSDREIQEL KQKIRLRRQQ IRTKNLLPAY RETENGSFRV TSSSSQFSSR DSTQLSESGS AEDADDLEIQ DADIRRSAVS NGKSSFSQNL SHCFLHSTSA EAVAMGLLKQ FEGMQLPAAS ELEWLVPEHD APQKLLPIPD SLPISPDDGQ HADIYKLRIR VRGNLEWAPP RPQIIFNVHP APTRKIAVAK QNYRCAGCGI RTDPDYIKRL RYCEYLGKYF CQCCHENAQM VVPSRILRKW DFSKYYVSNF SKDLLLKIWN DPLFNVQDIN SALYRKVKLL NQVRLLRVQL YHMKNMFKTC RLAKELLDSF DVVPGHLTED LHLYSLSDLT ATKKGELGPR LAELTRAGAA HVERCMLCQA KGFICEFCQN EEDVIFPFEL HKCRTCEECK ACYHKTCFKS GRCPRCERLQ ARRELLAKQS LESYLSDYEE EPTEALALEA TVLETT
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Characteristics
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    Expression System:

    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Endotoxin Level
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    Grade
    Crystallography grade
    Top Product
    Discover our top product KIAA0226 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    Rubicon (KIAA0226) (KIAA0226)
    Alternative Name
    Rubcn (KIAA0226 Products)
    Synonyms
    RUBICON Protein, 5330403K09 Protein, Kiaa0226 Protein, Rubicon Protein, mKIAA0226 Protein, RUN and cysteine rich domain containing beclin 1 interacting protein Protein, KIAA0226 Protein, RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein Protein, RUBCN Protein, KIAA0226 Protein, Rubcn Protein
    Background
    Run domain Beclin-1-interacting and cysteine-rich domain-containing protein (Rubicon),FUNCTION: Inhibits PIK3C3 activity, under basal conditions negatively regulates PI3K complex II (PI3KC3-C2) function in autophagy. Negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process (PubMed:19270693). Can sequester UVRAG from association with a class C Vps complex (possibly the HOPS complex) and negatively regulates Rab7 activation (By similarity). {ECO:0000250|UniProtKB:Q92622, ECO:0000269|PubMed:19270693}., FUNCTION: Involved in regulation of pathogen-specific host defense of activated macrophages. Following bacterial infection promotes NADH oxidase activity by association with CYBA thereby affecting TLR2 signaling and probably other TLR-NOX pathways. Stabilizes the CYBA:CYBB NADPH oxidase heterodimer, increases its association with TLR2 and its phagosome trafficking to induce antimicrobial burst of ROS and production of inflammatory cytokines. Following fungal or viral infection (implicating CLEC7A (dectin-1)-mediated myeloid cell activation or RIGI-dependent sensing of RNA viruses) negatively regulates pro-inflammatory cytokine production by association with CARD9 and sequestering it from signaling complexes (By similarity). {ECO:0000250|UniProtKB:Q92622}.
    Molecular Weight
    106.9 kDa
    UniProt
    Q80U62
    Pathways
    Autophagy
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