Plexin A4 Protein (PLXNA4) (AA 1258-1893) (His tag)

Catalog No. ABIN3136022

Product Details

Target: Plexin A4 (PLXNA4)

Protein Type: Recombinant

Protein Characteristics: AA 1258-1893

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This Plexin A4 protein is labelled with His tag.

Application: SDS-PAGE (SDS), Crystallization (Crys), ELISA, Western Blotting (WB)

Sequence: AYKRKSRESD LTLKRLQMQM DNLESRVALE CKEAFAELQT DIHELTSDLD GAGIPFLDYR TYTMRVLFPG IEDHPVLRDL EVPGYRQERV EKGLKLFAQL INNKVFLLSF IRTLESQRSF SMRDRGNVAS LIMTVLQSKL EYATDVLKQL LADLIDKNLE SKNHPKLLLR RTESVAEKML TNWFTFLLYK FLKECAGEPL FSLFCAIKQQ MEKGPIDAIT GEARYSLSED KLIRQQIEYK TLVLSCVSPD NVNSPEVPVK ILNCDTITQV KEKILDAIFK NVPCSHRPKA ADMDLEWRQG SGARMILQDE DITTKIENDW KRLNTVAHYQ VPDGSVVALV SKQVTAYNAV NNSTVSRTSA SKYENMIRYT GSPDSLRSRT PMITPDLESG VKLWHLVKNH EHGDQKEGDR GSKMVSEIYL TRLLATKGTL QKFVDDLFET IFSTAHRGSA LPLAIKYMFD FLDEQADKHG IHDPHVRHTW KSNCLPLRFW VNMIKNPQFV FDIHKNSITD ACLSVVAQTF MDSCSTSEHR LGKDSPSNKL LYAKDIPSYK NWVERYYSDI GKMPAISDQD MNAYLAEQSR MHMNEFNTMS ALSEIFSYVG KYSEEILGPL DHDDQCGKQK LAYKLEQVIT LMSLDS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Plxna4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Plexin A4 (PLXNA4)

Alternative Name: Plxna4 (PLXNA4 Products)

Synonyms: D204 Protein, wu:fd49b01 Protein, wu:fe15f03 Protein, PLXNA4A Protein, FAYV2820 Protein, PLEXA4 Protein, PLXNA4B Protein, PRO34003 Protein, 9330117B14 Protein, Plxa4 Protein, mKIAA1550 Protein, PLEX2 Protein, Plxna4 Protein, PLXNA4 Protein, plexin A4 Protein, plexin-A4 Protein, plxna4 Protein, PLXNA4 Protein, LOC100467706 Protein, Plxna4 Protein

Background: Coreceptor for SEMA3A. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance in the developing nervous system. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm. {ECO:0000269|PubMed:12591607, ECO:0000269|PubMed:18262512}.

Molecular Weight: 73.8 kDa Including tag.

UniProt: Q80UG2

Pathways: Regulation of Cell Size