Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

EIF4G3 Protein (AA 1-1579) (His tag)

Crystallography grade EIF4G3 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3136061
$24,004.89
Plus shipping costs $45.00 and $20.00 dry ice
Shipping to: United States
Will be delivered in 70 Business Days
  • Target See all EIF4G3 Proteins
    EIF4G3 (Eukaryotic Translation Initiation Factor 4 gamma 3 (EIF4G3))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-1579
    Origin
    Mouse
    Source
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This EIF4G3 protein is labelled with His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    MNSQPQARSP FFQRPQIQPP RAAIPNSSPS IRPGVQTPTA VYQANQHIMM VNHLPMPYPV TQGHQYCIPQ YRHSGPPYVG PPQQYPVQPP GPGPFYPGPG PGDFANAYGT PFYPSQPVYQ SAPIIVPTQQ QPPPAKREKK TIRIRDPNQG GKDITEEIMS GGGSRNPTPP IGRPASTPTP PQQLPSQVPE HSPVVYGTVE SAHLAASTPV TAASDQKQEE KPKPDPVFQS PSTVLRLVLS GEKKEQAGQM PETAAGEPTP EPPRTSSPTS LPPLARSSLP SPMSAALSSQ PLFTAEDKCE LPSSKEEDAP PVPSPTSCTA ASGPSLTDNS DICKKPCSVA PHDSQLISST ILINEMNGVG EKLSAKENTV GMLRQEVLPL TLELEILEHP QEELKVECTP TPIAPSMLPA FSPAPPTPPT SPPCPPVVLS AAIARSPAVA TEVQRVADEG ESLRTCLSKD AKEMQDKAES ESDGQAEETA DPQSLHSGRS PAPVQTATTA PKSWKKTKEQ TRTPDEVLEA EAEPKAEEEL AVDSVLEPEQ EKMSQGFPSE RDPSALKRGK AEEGNGEEAE PVRNGAESAS EGEGGDGNSG SADSSADGLT FPFKAESWKP ADTEGKKQYD REFLLDIQFM PACIQKPEGL PPISDVVLDK INQPRLSMRT LDPRILPRGP DFTPAFADFP RQTPGGRGVP LLNVGPRRSQ PGQRREPRKI ITVSVKEDVH LRKAENAWKP SQKRDSHADD PESIKTQELF RKVRSILNKL TPQMFNQLMK QVSALTVDTE ERLKGVIDLV FEKAIDEPSF SVAYANMCRC LVTLKVPMAD KPGNTVNFRK LLLNRCQKEF EKDKADDDVF EKKQKELEAA SAPEERTRLH DELEEAKDKA RRRSIGNIKF IGELFKLKML TEAIMHDCVV KLLKNHDEES LECLCRLLTT IGKDLDFEKA KPRMDQYFNQ MEKIVKERKT SSRIRFMLQD VIDLRLCNWV SRRADQGPKT IEQIHKEAKI EEQEEQRKVQ QLMTKEKRRP GVQRVDEGGW NTVQGAKNSR VLDPSKFLKI TKPTIDEKIQ LVPKAQLGSW GKGSSGGAKA SESDALRSSA SSLNRFSPLQ PPAPSGSPSA TPLEFDSRRA LTSRGSMGRE KSDKPIPAGT ARPNTFLRGS SKDLLDNQSQ EEQRREMLET VKQLTGGLDA ERASTEADRS KTRELAKSEM CAVPAPDKPA LSEEEVERKS KSIIDEFLHI NDFKEATQCI EELSAQGPLH VFVKVGVEFT LERSQITRDH MGHLLYQLVQ SEKLSKQDFF KGFSETLELA DDMAIDIPHI WLYLAELVTP MLKEGGISMR ELIVEFSKPL LPVGRAGVLL SEILHLLCRQ MSHKKVGALW READLSWKDF LPEGEDVHHF LLEQKLDFTE SEGPCSSEAL SKKELSAEEL SQRLEKLIME EKADDERIFD WVEANLDESQ MSSPTFLRAL MTAVCKAAII ADCSTFRVDT AVIKQRVPIL LKYLDSDTEK ELQALYALQA SIVKLDQPAN LLRMFFDCLY DEEVISEDAF YKWESSKDPA EQAGKGVALK SVTAFFTWLR EAEEESEDN
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Eif4g3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    EIF4G3 (Eukaryotic Translation Initiation Factor 4 gamma 3 (EIF4G3))
    Alternative Name
    Eif4g3 (EIF4G3 Products)
    Synonyms
    eif4g3 Protein, si:dkey-1a7.2 Protein, eIF-4G 3 Protein, eIF4G 3 Protein, eIF4GII Protein, 1500002J22Rik Protein, 4833436O05 Protein, 4930523M17Rik Protein, G1-419-52 Protein, repro8 Protein, eukaryotic translation initiation factor 4 gamma 3 Protein, eukaryotic translation initiation factor 4 gamma, 3 Protein, eukaryotic translation initiation factor 4 gamma, 3b Protein, eukaryotic translation initiation factor 4 gamma, 1 Protein, EIF4G3 Protein, CpipJ_CPIJ009979 Protein, eif4g3 Protein, eif4g3b Protein, eif4g1 Protein, Eif4g3 Protein
    Background
    Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1 (By similarity). {ECO:0000250}.
    Molecular Weight
    175.8 kDa Including tag.
    UniProt
    Q80XI3
You are here:
Support