Retinoblastoma Binding Protein 8 Protein (RBBP8) (AA 1-893) (Strep Tag)

Catalog No. ABIN3136083

Product Details

Target: Retinoblastoma Binding Protein 8 (RBBP8)

Protein Type: Recombinant

Protein Characteristics: AA 1-893

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This Retinoblastoma Binding Protein 8 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MSISGSGCGS PNSADASNDF KELWTKLKEY HDKEVQGLQV KVTKLKKERI LDAQRLEEFF TKNQQLRDQQ KVLQETIKIL EDRLRAGLCD RCAVTEEHMH KKQQEFENIR QQNLKLITEL MNEKNTLQEE NKKLSEQLQQ KMENGQQDQV AELACEENII PDSPVTSFSF SGINRLRKKE NLHVRYVEQT HTKLERSLCT NELRKISKDS APAPVNSEEH EILVADTCDQ NHSPLSKICE TSSYPTDKTS FNLDTVVAET LGLNGQEESE PQGPMSPLGS ELYHCLKEDH KKHPFMESAR SKEDSLRFSD SASKTPPQEF TTRASSPVFG ATSTVKAHLG LNTSFSPSLL DIGKKNLLKT APFSNIAVSR SEKVRSKSED NALFTQHSLG SEVKVISQSF SSKQILTNKT VSDSVDEQCS ADHMNTTVAD KYLVPLKSLG GKASKRKRTE EESEHAVKCP QACFDKENAL PFPMENQFSM NGDHVMDKPL DLSDRFAATQ RQEKNHGNET SKNKLKQATI YEALKPIPKG SSSGRKALSG DCMPAKDSWE TYCLQPRSLQ SSSKFSPDQK TPLQIKEENP VFKTPPCSQE SLETENLFGD VKGTGSLVPT KVKSRAVHGG CELASVLQLN PCRVAKTKAL PSNQDTSFEN IQWSVDPGAD LSQYKMDVTV IDTKDSSHSR LGGETVDMDC TLVSETVLLK MKKQEQKERS PNGDIKMNDS LEDMFDRTTH EEYESCLADS FSQVPDEEEL PDTTKKTNIP ADKQDGVKQK AFVGPYFKDK ERETSIQNFP HIEVVRKKEE RRKLLGHTCK ECEIYYADLP AEEREKKLAS CSRHRFRYIP PNTPENFWEV GFPSTQTCLE RGYIKEDLDL SPRPKRRQPY NAVFSPKGKE QRT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Retinoblastoma Binding Protein 8 (RBBP8)

Alternative Name: Rbbp8 (RBBP8 Products)

Synonyms: COM1 Protein, CTIP Protein, JWDS Protein, RIM Protein, SAE2 Protein, SCKL2 Protein, 9930104E21Rik Protein, CtIP Protein, RBBP-8 Protein, RGD1308872 Protein, RBBP8 Protein, DKFZp469K0714 Protein, RB binding protein 8, endonuclease Protein, retinoblastoma binding protein 8, endonuclease Protein, retinoblastoma binding protein 8 L homeolog Protein, retinoblastoma binding protein 8 Protein, RBBP8 Protein, Rbbp8 Protein, rbbp8.L Protein, rbbp8 Protein

Background: DNA endonuclease RBBP8 (EC 3.1.-.-) (CtBP-interacting protein) (CtIP) (Retinoblastoma-binding protein 8) (RBBP-8) (Retinoblastoma-interacting protein and myosin-like) (RIM) (Sporulation in the absence of SPO11 protein 2 homolog) (SAE2),FUNCTION: Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double-strand break (DSB) repair through the homologous recombination (HR) pathway (By similarity). HR is restricted to S and G2 phases of the cell cycle and preferentially repairs DSBs resulting from replication fork collapse (By similarity). Key determinant of DSB repair pathway choice, as it commits cells to HR by preventing classical non-homologous end-joining (NHEJ) (By similarity). Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA (By similarity). Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage (By similarity). During immunoglobulin heavy chain class-switch recombination, promotes microhomology-mediated alternative end joining (A-NHEJ) and plays an essential role in chromosomal translocations (PubMed:21131978, PubMed:21131982). Binds preferentially to DNA Y-junctions and to DNA substrates with blocked ends and promotes intermolecular DNA bridging (By similarity). {ECO:0000250|UniProtKB:Q99708, ECO:0000269|PubMed:21131978, ECO:0000269|PubMed:21131982}.

Molecular Weight: 100.8 kDa

UniProt: Q80YR6

Pathways: Cell Division Cycle, DNA Damage Repair