TXNIP Protein (AA 1-397) (Strep Tag)

Catalog No. ABIN3136127

Product Details

Target: TXNIP (Thioredoxin Interacting Protein (TXNIP))

Protein Type: Recombinant

Protein Characteristics: AA 1-397

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This TXNIP protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MVMFKKIKSF EVVFNDPEKV YGSGEKVAGR VIVEVCEVTR VKAVRILACG VAKVLWMQGS QQCKQTLDYL RYEDTLLLEE QPTAGENEMV IMRPGNKYEY KFGFELPQGP LGTSFKGKYG CVDYWVKAFL DRPSQPTQEA KKNFEVMDLV DVNTPDLMAP VSAKKEKKVS CMFIPDGRVS VSARIDRKGF CEGDDISIHA DFENTCSRIV VPKAAIVARH TYLANGQTKV FTQKLSSVRG NHIISGTCAS WRGKSLRVQK IRPSILGCNI LKVEYSLLIY VSVPGSKKVI LDLPLVIGSR SGLSSRTSSM ASRTSSEMSW IDLNIPDTPE APPCYMDIIP EDHRLESPTT PLLDDVDDSQ DSPIFMYAPE FQFMPPPTYT EVDPCVLNNN NNNNNVQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: TXNIP (Thioredoxin Interacting Protein (TXNIP))

Alternative Name: Txnip (TXNIP Products)

Synonyms: EST01027 Protein, HHCPA78 Protein, THIF Protein, VDUP1 Protein, Gm348 Protein, Trf3 Protein, TBP2 Protein, TRF3 Protein, 1200008J08Rik Protein, AA682105 Protein, Hyplip1 Protein, Tbp-2 Protein, Vdup1 Protein, sb:cb368 Protein, txnip Protein, thioredoxin interacting protein Protein, TATA box binding protein like 2 Protein, TATA-box binding protein like 2 Protein, thioredoxin interacting protein L homeolog Protein, thioredoxin interacting protein a Protein, TXNIP Protein, Tbpl2 Protein, TBPL2 Protein, Txnip Protein, txnip.L Protein, txnip Protein, txnipa Protein

Background: Thioredoxin-interacting protein (Vitamin D3 up-regulated protein 1),FUNCTION: May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1) (By similarity). Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth. {ECO:0000250, ECO:0000269|PubMed:15723808, ECO:0000269|PubMed:15930262}.

Molecular Weight: 44.4 kDa

UniProt: Q8BG60

Pathways: Protein targeting to Nucleus, Platelet-derived growth Factor Receptor Signaling, Inflammasome