MIA3 Protein (AA 1224-1930) (His tag)

Catalog No. ABIN3136176

Product Details

Target: MIA3 (Melanoma Inhibitory Activity Family, Member 3 (MIA3))

Protein Type: Recombinant

Protein Characteristics: AA 1224-1930

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This MIA3 protein is labelled with His tag.

Application: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: SWRTILVVKS RVYQVTEKQI SEKLENIKKE NAELMQKLSS YEQKIKESKK YVQETKKQNM ILSDEAVKYK DKIKILEETN VSLGDKAKSL RLQLESEREQ NVKNQDLILE NKKSIEKLKD VISMNASELS EVQVALNEAK LSEENVKSEC HRVQEENARL KKKKEQLQQQ VEEWSKSHAE LTGQIKSFEK SQEDLEIALT HKDDNISALT NCITQLNRLE CELESEDPDK GGNESDDLAN GETGGDRSEK IRNRIKQMMD VSRTQTAVSI VEEDLKLLQL KLRASMSTKC NLEDQIKKLE DDRSSLQTAK AGLEDECKTL RQKVEILNEL YQQKEMALQK KLSQEEYERQ DREQRLTAAD EKVVLAAEEV KTYKRRIEEM EEELQKTERS FKNQIAAHEK KAHDNWLKAR AAERAMAEEK REAANLRHKL LEMTQKMAMR QDEPVIVKPM PGRPNTQNPP RRGLLSQNGS FGPSPVSGGE CSPPLPAEPP GRPLSATLSR RDTPRSEFGS LDRHLPRPRW PSEASGKHSA SDPGPAPVVN SSSRSSSPAK AVDEGKVNMA PKGPPPFPGV PLMGGPVPPP IRYGPPPQLC GGPFGPRPLP PPFVPGMHPP LGVREYAPGV LPGKRDLPLD PREFLPGHTP FRPPGSLGPR EFFIPGTRLP PPTHGPQEYP PPPPAVRDSL PSGPREEAKP ASPSSVQDRS QASKPTP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Mia3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: MIA3 (Melanoma Inhibitory Activity Family, Member 3 (MIA3))

Alternative Name: Mia3 (MIA3 Products)

Synonyms: CG11098 Protein, Dmel\\CG11098 Protein, TANGO1 Protein, MIA3 Protein, 9130229H14Rik Protein, A930039G15Rik Protein, AU022327 Protein, B230399H06Rik Protein, C80126 Protein, Gm1525 Protein, Tango Protein, ARNT Protein, D320 Protein, TANGO Protein, UNQ6077 Protein, Transport and Golgi organization 1 Protein, MIA family member 3, ER export factor Protein, melanoma inhibitory activity family, member 3 Protein, melanoma inhibitory activity protein 3 Protein, melanoma inhibitory activity family member 3 Protein, melanoma inhibitory activity 3 Protein, Tango1 Protein, MIA3 Protein, LOC100055507 Protein, mia3 Protein, Mia3 Protein

Background: Required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. May participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1 (By similarity). {ECO:0000250}.

Molecular Weight: 80.2 kDa Including tag.

UniProt: Q8BI84