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ARID5B Protein (AA 1-1188) (His tag)

Crystallography grade ARID5B Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3136224
  • Target See all ARID5B Proteins
    ARID5B (AT Rich Interactive Domain 5B (MRF1-Like) (ARID5B))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-1188
    Origin
    Mouse
    Source
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This ARID5B protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    MEPNSLQWVG SPCGLHGPYI FYKAFQFHLE GKPRILSLGD FFFVRCTPKD PICIAELQLL WEERTSRQLL SSSKLYFLPE DTPQGRNSDH GEDEVIAVSE KVIVKLEDLV KWAHSDFSKW RCGLRATPVK TEAFGRNGQK EALLRYRQST LNSGLNFKDV LKEKADLGED EEETNVIVLS YPQYCRYRSM LKRIQDKPSS ILTDQFALAL GGIAVVSRNP QILYCRDTFD HPTLIENESV CDEFAPNLKG RPRKKKTCPQ RRDSFSGSKD PNNNCDGKVI SKVKGEARSA LTKPKNNHNN CKKTSNEEKP KLSIGEECRA DEQAFLVALY KYMKERKTPI ERIPYLGFKQ INLWTMFQAA QKLGGYETIT ARRQWKHIYD ELGGNPGSTS AATCTRRHYE RLILPYERFI KGEEDKPLPP IKPRKQENNT QENENKTKVS GNKRIKQEMA KNKKEKENTP KPQDTSEVSS EQRKEEETLN HKSAPEPLPA PEVKGKPEGH KDLGARAPVS RADPEKANET DQGSNSEKEA EEMGDKGLAP LLPSPPLPPE KDSAPTPGAG KQPLASPSTQ MDSKQEAKPC CFTESPEKDL QGAPFSSFSA TKPPLTSQNE AEEEQLPATA NYIANCTVKV DQLGSDDIHT ALKQTPKVLV VQSFDMFKDK DLTGPMNENH GLNYTPLLYS RGNPGIMSPL AKKKLLSQVS GASLSSSYPY GSPPPLISKK KLIAREDLCS GLSQGHHSQS SDHTAVSRPS VIQHVQSFKN KASEDRKSIN DIFKHDKLSR SDAHRCGFSK HQLGSLADSY ILKQETQEGK DKLLEKRAVS HAHVPSFLAD FYSSPHLHSL YRHTEHHLHN EQSSKYAARD AYQESENGAF LSHKHPEKIH VNYLASLHLQ DKKVAAAEAS TDDQPTDLSL PKNPHKLTSK VLGLAHSTSG SQEIKGASQF QVVSNQSRDC HPKACRVSPM TMSGPKKYPE SLARSGKPHQ VRLENFRKME GMVHPILHRK MSPQNIGAAR PIKRSLEDLD LVIAGKKARA VSPLDPAKEA SGKEKASEQE SEGNKGAYGG HSGAASEGHK LPLSTPIFPG LYSGSLCNSG LNSRLPAGYS HSLQYLKNQT VLSPLMQPLA FHSLVMQRGI FTSPTNSQQL YRHLAAATPV GSSYGDLLHN SIYPLAGINP QAAFPSSQLS SVHPSTKL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Arid5b Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product ARID5B Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    ARID5B (AT Rich Interactive Domain 5B (MRF1-Like) (ARID5B))
    Alternative Name
    Arid5b (ARID5B Products)
    Synonyms
    ARID5B Protein, DKFZp469H121 Protein, DKFZP469L1210 Protein, DESRT Protein, MRF-2 Protein, MRF2 Protein, 4930580B11 Protein, 5430435G07Rik Protein, AI467247 Protein, Desrt Protein, Mrf2 Protein, Mrf2alpha Protein, Mrf2beta Protein, AT-rich interaction domain 5B Protein, AT-rich interaction domain 5B S homeolog Protein, AT rich interactive domain 5B (MRF1-like) Protein, ARID5B Protein, arid5b.S Protein, Arid5b Protein
    Background
    Transcription coactivator that binds to the 5'-AATA[CT]-3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-337, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver (By similarity). Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation. {ECO:0000250, ECO:0000269|PubMed:12215486, ECO:0000269|PubMed:14651970, ECO:0000269|PubMed:17962384, ECO:0000269|PubMed:18070594, ECO:0000269|PubMed:19913508}.
    Molecular Weight
    132.8 kDa Including tag.
    UniProt
    Q8BM75
    Pathways
    Platelet-derived growth Factor Receptor Signaling
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