DNAAF2 Protein (AA 1-814) (His tag)
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- Target See all DNAAF2 (C14orf104) Proteins
- DNAAF2 (C14orf104) (Chromosome 14 Open Reading Frame 104 (C14orf104))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-814
- Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This DNAAF2 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA
- Sequence
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MAKAAASSAL EDLDLSREEV QRFTSAFQDP EFRRMFSEYA AEITDPENRR RYEEEITALE RERGVDVRFV HPEPGHVLRT SLDGEHRCYV NVCSNSLVGV PSSRPGPGRG GTAAGSHWSL PYSLAPGRQY AGRNGARYTV YDVVFHPEAL ALARSHERFR EMLDATALEA VEQQFGVRLD RRNAKTLKIK YKGMPEAAVL RTPLPGGVPA QPEGEPPGLF PDPPYPYRYP AAAAANTARS PASPAPEAVQ RPEPTEPRCS VVQRHHVDLQ DYRCSRDAAP STVPHELVVT IELPLLRSVE RAELEVKGKL LCLDSRNPDY RLRLSLPYPV DDGRGKAQYN KARRQLVVTL PVALADARQE PPAATPEEPA EETGTDDVAR TSAGDFAAAR EESADGTGAD HGEKSGVGAP DPGAAHAEGE LVPEPEQDFG GDSVAPLDLG KGTSPGDRSL PYSAFPGGDT ESLCGDPGVQ TNEEQERTRH DTAGSAMGDP GTESIAPVCP PLQCNQDEDS LTLLIQVPGI LPQTLHGHLS PVGYELCFST QDSGYSCTLQ FAPENKLSTR EPETSVSLNN AVIVLAKSPE SHGLWREWYC GLNKESLEER LFINEENVNG FLEEVLCSPL KQARSLAPPL IEVLQATDEQ VQIHAELQEC SDPAGLQGKG KGVREGCPLS EAEAADQSAT SPAASDSAAA VEALKINTHG SAVDLQHGCP EVPHVLSGKP LQPEAKMDPE FIRESSTTYS TEEKENIREP VISKGEKING DHPSSLLNKT VVQNIPDFDT IKETNMQDGS VQIIRDHTTH CAFSFQNPLL YDLD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Dnaaf2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product C14orf104 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- DNAAF2 (C14orf104) (Chromosome 14 Open Reading Frame 104 (C14orf104))
- Alternative Name
- Dnaaf2 (C14orf104 Products)
- Synonyms
- 1110034A24Rik Protein, 2810020C19Rik Protein, AV032410 Protein, Ktu Protein, TU54 Protein, mknt Protein, C14orf104 Protein, CILD10 Protein, KTU Protein, PF13 Protein, C10H14orf104 Protein, RGD1310311 Protein, c14orf104 Protein, cild10 Protein, kintoun Protein, ktu Protein, zgc:110294 Protein, dynein axonemal assembly factor 2 Protein, dynein, axonemal assembly factor 2 Protein, dynein, axonemal, assembly factor 2 Protein, dynein, axonemal, assembly factor 2 L homeolog Protein, DNAAF2 Protein, Dnaaf2 Protein, dnaaf2.L Protein, dnaaf2 Protein
- Background
- Required for cytoplasmic pre-assembly of axonemal dyneins, thereby playing a central role in motility in cilia and flagella. Involved in pre-assembly of dynein arm complexes in the cytoplasm before intraflagellar transport loads them for the ciliary compartment (By similarity). {ECO:0000250}.
- Molecular Weight
- 89.3 kDa Including tag.
- UniProt
- Q8BPI1
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