Polybromo 1 Protein (PBRM1) (AA 1-1634) (Strep Tag)

Catalog No. ABIN3136260

Product Details

Target: Polybromo 1 (PBRM1)

Protein Type: Recombinant

Protein Characteristics: AA 1-1634

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This Polybromo 1 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MGSKRRRATS PSSSVSGDFD DGHHSVPTPG PSRKRRRLSN LPTVDPIAVC HELYNTIRDY KDEQGRLLCE LFIRAPKRRN QPDYYEVVSQ PIDLMKIQQK LKMEEYDDVN LLTADFQLLF NNAKAYYKPD SPEYKAACKL WDLYLRTRNE FVQKGEADDE DDDEDGQDNQ GTLADGSSPG YLKEILEQLL EAIVVATNPS GRLISELFQK LPSKVQYPDY YAIIKEPIDL KTIAQRIQNG SYKSIHAMAK DIDLLAKNAK TYNEPGSQVF KDANSIKKIF YMKKAEIEHH EMTKSSLRIR TASNLAAARL TGPSHNKSSL GEERNPTSKY YRNKRAVQGG RLSAITMALQ YGSESEEDAA LAAARYEEGE SEAESITSFM DVSNPFHQLY DTVRSCRNHQ GQLIAEPFFH LPSKKKYPDY YQQIKMPISL QQIRTKLKNQ EYETLDHLEC DLNLMFENAK RYNVPNSAIY KRVLKLQQVM QAKKKELARR DDIEDGDSMI SSATSDTGSA KRKSKKNIRK QRMKILFNVV LEAREPGSGR RLCDLFMVKP SKKDYPDYYK IILEPMDLKI IEHNIRNDKY AGEEGMMEDM KLMFRNARHY NEEGSQVYND AHILEKLLKD KRKELGPLPD DDDMASPKLK LSRKSGVSPK KSKYMTPMQQ KLNEVYEAVK NYTDKRGRRL SAIFLRLPSR SELPDYYLTI KKPMDMEKIR SHMMANKYQD IDSMVEDFVM MFNNACTYNE PESLIYKDAL VLHKVLLETR RDLEGDEDSH VPNVTLLIQE LIHNLFVSVM SHQDDEGRCY SDSLAEIPAV DPNSPNKPPL TFDIIRKNVE SNRYRRLDLF QEHMFEVLER ARRMNRTDSE IYEDAVELQQ FFIRIRDELC KNGEILLSPA LSYTTKHLHN DVEKEKKEKL PKEIEEDKLK REEEKREAEK SEDSSGTTGL SGLHRTYSQD CSFKNSMYHV GDYVYVEPAE ANLQPHIVCI ERLWEDSAGE KWLYGCWFYR PNETFHLATR KFLEKEVFKS DYYNKVPVSK ILGKCVVMFV KEYFKLCPEN FRDEDVFVCE SRYSAKTKSF KKIKLWTMPI SSVRFVPRDV PLPVVRVASV FANADKGDDE KNTDNSDDNR AEDNFNLEKE KEDVPVEMSN GEPGCHYFEQ LRYNDMWLKV GDCVFIKSHG LVRPRVGRIE KVWVRDGAAY FYGPIFIHPE ETEHEPTKMF YKKEVFLSNL EETCPMSCIL GKCAVLSFKD FLSCRPTEIP ENDILLCESR YNESDKQMKK FKGLKRFSLS AKVVDDEIYY FRKPIIPQKE PSPLLEKKIQ LLEAKFAELE GGDDDIEEMG EEDSEVIEAP SLPQLQTPLA NELDLMPYTP PQSTPKSAKG SAKKESSKRK INMSGYILFS SEMRAVIKAQ HPDYSFGELS RLVGTEWRNL ETAKKAEYEG MMGGYPPGLP PLQGPVDGLV SMGSMQPLHP GGPPPHHLPP GVPGLPGIPP PGVMNQGVAP MVGTPAPGGS PYGQQVGVLG PPGQQAPPPY PGPHPAGPPV IQQPTTPMFV APPPKTQRLL HSEAYLKYIE GLSAESNSIS KWDQTLAARR RDVHLSKEQE SRLPSHWLKS KGAHTTMADA LWRLRDLMLR DTLNIRQAYN LENV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Polybromo 1 (PBRM1)

Alternative Name: Pbrm1 (PBRM1 Products)

Synonyms: pb1 Protein, BAF180 Protein, PB1 Protein, 2610016F04Rik Protein, AI507524 Protein, Pb1 Protein, RGD1565549 Protein, polybromo 1 Protein, polybromo 1 L homeolog Protein, protein polybromo-1 Protein, PBRM1 Protein, pbrm1.L Protein, LOC100170602 Protein, pbrm1 Protein, Pbrm1 Protein

Background: Protein polybromo-1 (BRG1-associated factor 180) (BAF180),FUNCTION: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). Acts as a negative regulator of cell proliferation. {ECO:0000250|UniProtKB:Q86U86, ECO:0000303|PubMed:22952240, ECO:0000303|PubMed:26601204}.

Molecular Weight: 187.2 kDa

UniProt: Q8BSQ9