SALL4 Protein (AA 1-1067) (His tag)
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- Target See all SALL4 Proteins
- SALL4 (Sal-Like 4 (SALL4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1067
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Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This SALL4 protein is labelled with His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- Sequence
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MSRRKQAKPQ HINWEEGQGE QPQQLPSPDL AEALAAEEPG APVNSPGNCD EASEDSIPVK RPRREDTHIC NKCCAEFFSL SEFMEHKKSC TKTPPVLIMN DSEGPVPSED FSRAALSHQL GSPSNKDSLQ ENGSSSGDLK KLGTDSILYL KTEATQPSTP QDISYLPKGK VANTNVTLQA LRGTKVAVNQ RGAEAPMAPM PAAQGIPWVL EQILCLQQQQ LQQIQLTEQI RVQVNMWAAH ALHSGVAGAD TLKALSSHVS QQVSVSQQVS AAVALLSQKA SNPALSLDAL KQAKLPHASV PSAASPLSSG LTSFTLKPDG TRVLPNFVSR LPSALLPQTP GSVLLQSPFS AVTLDQSKKG KGKPQNLSAS ASVLDVKAKD EVVLGKHKCR YCPKVFGTDS SLQIHLRSHT GERPYVCPIC GHRFTTKGNL KVHLQRHPEV KANPQLLAEF QDKGAVSAAS HYALPVPVPA DESSLSVDAE PVPVTGTPSL GLPQKLTSGP NSRDLMGGSL PNDMQPGPSP ESEAGLPLLG VGMIHNPPKA GGFQGTGAPE SGSETLKLQQ LVENIDKATT DPNECLICHR VLSCQSSLKM HYRTHTGERP FQCKICGRAF STKGNLKTHL GVHRTNTTVK TQHSCPICQK KFTNAVMLQQ HIRMHMGGQI PNTPLPESPC DFTAPEPVAV SENGSASGVC QDDAAEGMEA EEVCSQDVPS GPSTVSLPVP SAHLASPSLG FSVLASLDTQ GKGALPALAL QRQSSRENSS LEGGDTGPAN DSSLLVGDQE CQSRSPDATE TMCYQAVSPA NSQAGSVKSR SPEGHKAEGV ESCRVDTEGR TSLPPTFIRA QPTFVKVEVP GTFVGPPSMP SGMPPLLASQ PQPRRQAKQH CCTRCGKNFS SASALQIHER THTGEKPFVC NICGRAFTTK GNLKVHYMTH GANNNSARRG RKLAIENPMA ALSAEGKRAP EVFSKELLSP AVSVDPASWN QYTSVLNGGL AMKTNEISVI QSGGIPTLPV SLGASSVVSN GTISKLDGSQ TGVSMPMSGN GEKLAVPDGM AKHQFPHFLE ENKIAVS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Sall4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product SALL4 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- SALL4 (Sal-Like 4 (SALL4))
- Alternative Name
- Sall4 (SALL4 Products)
- Synonyms
- SALL4 Protein, drrs Protein, sall4a Protein, znf797 Protein, MGC81541 Protein, hsal4 Protein, MGC76059 Protein, cb614 Protein, sb:cb372 Protein, sb:cb614 Protein, wu:fa01g03 Protein, ik:tdsubc_2c1 Protein, xx:tdsubc_2c1 Protein, 5730441M18Rik Protein, AA407717 Protein, AL022809 Protein, AW536104 Protein, C330011P20Rik Protein, C78083 Protein, C78563 Protein, Tex20 Protein, DRRS Protein, HSAL4 Protein, ZNF797 Protein, dJ1112F19.1 Protein, spalt like transcription factor 4 Protein, sal-like 4 (Drosophila) Protein, spalt-like transcription factor 4 L homeolog Protein, spalt-like transcription factor 4 Protein, SALL4 Protein, sall4 Protein, sall4.L Protein, Sall4 Protein
- Background
- Transcription factor with a key role in the maintenance and self-renewal of embryonic and hematopoietic stem cells. {ECO:0000250}.
- Molecular Weight
- 114.1 kDa Including tag.
- UniProt
- Q8BX22
- Pathways
- Stem Cell Maintenance, Tube Formation
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