CADPS2 Protein (AA 1-1297) (Strep Tag)

Catalog No. ABIN3136322

Product Details

Target: CADPS2 (Ca++-Dependent Secretion Activator 2 (CADPS2))

Protein Type: Recombinant

Protein Characteristics: AA 1-1297

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This CADPS2 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MLDPSSSEEE SDEGLEEESR EVLVAPGVSQ RAPPAAAREG RRDAPGRSGG GSGGGAARPV SPSPSVLSEG RNEPELQLDE EQERRIRLQL YVFVVRCIAY PFNAKQPTDM ARRQQKLNKQ QLQLLKERFQ AFLNGETQIV ADEAFCNAVR SYYEVFLKSD RVARMVQSGG CSANDFREVF KKNIEKRVRS LPEIDGLSKE TVLSSWIAKY DAIYRGEEDL CKQPNRMTLS AVSELILSKE QLYEMFQQIL GIKKLEHQLL YNACQLDNAD EQAAQIRREL DGRLQLAEKM AKERRFPRFI SKEMESMYIE ELRASVNLLM ANLESLPVSK GGPEFKLQKL KRSQNSAFLD LGDENEIQLS KSDVVLSFTL EIVIMEVQGL KSVAPNRIVY CTMEVEGGEK LQTDQAEASR PQWGTQGDFN TTHPRPVVKV KLFTESTGVL ALEDKELGRV VLYPTSNSSK SAELHRMTVP KNSQDSDLKI KLAVRMDKPA HMKHSGYLYA LGQKVWKRWK KRYFVLVQVS QYTFAMCSYR EKKSEPQELM QLEGYTVDYT DPHPGLQGGQ VFFNAVKEGD TVIFASDDEQ DRILWVQAMY RATGQSYKPV PAVQSQKLNP KGGALHADAQ LYADRFQKHG MDEFISASPC KLDHAFLFRI LQRQTLDHRL NDSYSCLGWF SPGQVFVLDE YCARYGVRGC HRHLCYLTEL MEHSENGAVI DPTLLHYSFA FCASHVHGNR PDGIGTVSVE EKERFEEIKD RLSSLLENQI SHFRYCFPFG RPEGALKATL SLLERVLMKD IATPIPAEEV KKVVRKCLEK AALINYTRLT EYAKIEETMN QATPARKLEE VLHLAELCIE VLQQNEEHHA EGREAFAWWP DLLAEHAEKF WALFTVDMDT ALEAQPQDSW DSFPLFQLLN NFLRNDTLLC NGKFHKHLQE IFVPLVVRYV DLMESAIAQS IHRGFEQETW QPVKNIANSL PNVALPKVPS LPLNLPQIPS FSTPPWMASL YESTNGSTTS EDLFWKLDAL QMFVFDLHWP EQEFAHHLEQ RLKLMASDMI EACVKRTRTA FELKLQKANK TTDLRIPASV CTMFNVLVDA KKQSTKLCAL DGGQEQQYHS KIDDLIDNTV KEIIALLVSK FVSVLEGVLS KLSRYDEGTF FSSILSFTVK AAAKYVDVPK PGMDLADTYI MFVRQNQDIL REKVNEEMYI EKLFDQWYSN SMKVICVWLA DRLDLQLHIY QLKTLIKIVK KTYRDFRLQG VLEGTLNSKT YDTLHRRLTV EEATASVSEG GGLQGITMKD SDEEEEG
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: CADPS2 (Ca++-Dependent Secretion Activator 2 (CADPS2))

Alternative Name: Cadps2 (CADPS2 Products)

Synonyms: RGD1559440 Protein, A230044C21Rik Protein, Caps-2 Protein, Caps2 Protein, Cpd2 Protein, caps Protein, cpd2 Protein, zgc:110135 Protein, calcium dependent secretion activator 2 Protein, Ca2+-dependent activator protein for secretion 2 Protein, Ca++-dependent secretion activator 2 Protein, CADPS2 Protein, Cadps2 Protein, cadps2 Protein

Background: Calcium-dependent secretion activator 2 (Calcium-dependent activator protein for secretion 2) (CAPS-2),FUNCTION: Calcium-binding protein involved in exocytosis of vesicles filled with neurotransmitters and neuropeptides. Probably acts upstream of fusion in the biogenesis or maintenance of mature secretory vesicles. Regulates neurotrophin release from granule cells leading to regulate cell differentiation and survival during cerebellar development. May specifically mediate the Ca(2+)-dependent exocytosis of large dense-core vesicles (DCVs) and other dense-core vesicles.

Molecular Weight: 147.8 kDa

UniProt: Q8BYR5

Pathways: Synaptic Vesicle Exocytosis