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Ephrin A1 Protein (EFNA1) (AA 1-1127) (His tag)

Crystallography grade EFNA1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Catalog No. ABIN3136341
  • Target See all Ephrin A1 (EFNA1) Proteins
    Ephrin A1 (EFNA1)
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-1127
    Origin
    • 15
    • 3
    • 3
    • 1
    • 1
    Mouse
    Source
    • 6
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This Ephrin A1 protein is labelled with His tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Sequence
    MVLSGVDKMI RLQKNTANIR NICVLAHVDH GKTTLADCLI SSNGIISSRL AGKLRYMDSR EDEQVRGITM KSSAISLHYA EGHEEYLINL IDSPGHVDFS SEVSTAVRIC DGCIIVVDAV EGVCPQTQAV LRQAWLENIR PVLVINKIDR LIVELKFTPQ EAYSHLKNIL EQINALTGTL FTSKVLEERA ERETESQAKP HSEQGEQVYD WSAGLEDVDD SQLYFSPEQG NVVFTSAIDG WGFGIEHFAR IYSQKIGIKK EVLLKTLWGD YYINMKAKKI MKVDQAKGKK PLFVQLILEN IWSLYDAVLK KDKEKIDKIV TSLGLKIGAR EARHSDPKVQ INAICSQWLP ISHAVLAMVC HKLPSPLDMT SERVEKLLCT GSQTFESLPP ETQALKAAFM KCGSEDTAPV IIFVSKMFAV DVKALPQNKP RPLTQEEMAQ RRERARQRHA EKLAAAQGQT SQGPTQDGGA LETSPHEDEP RGDEPDVASV SRQPVSQEES SQEAFIAFAR VFSGIARRGK KIFVLGPKYS PVDFLQRVPL GFSAPLEDLP PVPHMACCTL ENLYLLMGRE LEDLEEVPPG NVLGIGGLQD FVLKSATLCS LPSCPPFIPL NFEATPIVRV AVEPKHPSEM PQLVKGMKLL NQADPCVQVL IQETGEHVLV TAGEVHLQRC LDDLRERFAK IHISVSEPII PFRETITKPP KVDMVNEEIG RQQKVAVIHQ TKEEQSKIPE GIHVDSDGLI TIPTPNKLAT LSVRAIPLPE EVTRILEENS DLIRSMELLT SSLNEGRNTQ AIHQKTQEKI WEFKGKLEKH LTGRKWRNTV DQIWSFGPRK CGPNILVSRS EDFQNSVWSG PAGRESKEAS RFRDFGNSIV SGFQLATLSG PMCEEPLMGV CFVLEKWELN KCAEQGASDK QHQGQCDLAG EGQGGGKTCH VGDENQEQQD VCSEPFEETS QKGDSPVIDC YGPFSGQLIA TMKEACRYAL QVKPQRLMAA MYTCDIMATS DVLGRVYAVL SKREGRVLQE EMKEGTDMFI IKAVLPVAES FGFADEIRKR TSGLASPQLV FSHWEVIPSD PFWVPTTEEE YLHFGEKADS ENQARKYMNA VRKRKGLYVE EKIVEHAEKQ RTLSKNK
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Efl1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product EFNA1 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    Ephrin A1 (EFNA1)
    Alternative Name
    Efl1 (EFNA1 Products)
    Synonyms
    Xefrin-A1 Protein, b61 Protein, ecklg Protein, efl1 Protein, efna1 Protein, efna1a Protein, elfa-a Protein, epha1 Protein, eplg1 Protein, lerk1 Protein, tnfaip4 Protein, xelf-a' Protein, xelfa Protein, AI325262 Protein, B61 Protein, Efl1 Protein, Epl1 Protein, Eplg1 Protein, Lerk1 Protein, ECKLG Protein, EFL1 Protein, EPLG1 Protein, LERK-1 Protein, LERK1 Protein, TNFAIP4 Protein, efna1b Protein, MGC130661 Protein, ephrin-A1 S homeolog Protein, ephrin-A1 Protein, ephrin A1 Protein, ephrin-A1b Protein, ephrin-A1 L homeolog Protein, efna1.S Protein, efna1 Protein, EFNA1 Protein, efna1b Protein, Efna1 Protein, efna1.L Protein
    Background
    Involved in the biogenesis of the 60S ribosomal subunit and translational activation of ribosomes. Together with SBDS, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Has low intrinsic GTPase activity. GTPase activity is increased by contact with 60S ribosome subunits (By similarity). {ECO:0000250}.
    Molecular Weight
    126.7 kDa Including tag.
    UniProt
    Q8C0D5
    Pathways
    RTK Signaling
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