IKZF4 Protein (AA 1-586) (Strep Tag)

Catalog No. ABIN3136362

Product Details

Target: IKZF4 (IKAROS Family Zinc Finger 4 (Eos) (IKZF4))

Protein Type: Recombinant

Protein Characteristics: AA 1-586

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This IKZF4 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MHTPPALPRR FQGGGRVRTP GSHRQGKDNL ERELSGGCAP DFLPQAQDSN HFIMESLFCE SSGDSSLEKE FLGAPVGPSV STPNSQHSSP SRSLSANSIK VEMYSDEESS RLLGPDERLL DKDDSVIVED SLSEPLGYCD GSGPEPHSPG GIRLPNGKLK CDVCGMVCIG PNVLMVHKRS HTGERPFHCN QCGASFTQKG NLLRHIKLHS GEKPFKCPFC NYACRRRDAL TGHLRTHSVS SPTVGKPYKC NYCGRSYKQQ STLEEHKERC HNYLQSLSTD AQALTGQPGD EIRDLEMVPD SMLHPSTERP TFIDRLANSL TKRKRSTPQK FVGEKQMRFS LSDLPYDVNA SGGYEKDVEL VAHHGLEPGF GGSLAFVGTE HLRPLRLPPT NCISELTPVI SSVYTQMQPI PSRLELPGSR EAGEGPEDLG DGGPLLYRAR GSLTDPGASP SNGCQDSTDT ESNHEDRIGG VVSLPQGPPP QPPPTIVVGR HSPAYAKEDP KPQEGLLRGT PGPSKEVLRV VGESGEPVKA FKCEHCRILF LDHVMFTIHM GCHGFRDPFE CNICGYHSQD RYEFSSHIVR GEHKVG
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: IKZF4 (IKAROS Family Zinc Finger 4 (Eos) (IKZF4))

Alternative Name: Ikzf4 (IKZF4 Products)

Synonyms: A630026H08Rik Protein, Eos Protein, Zfpn1a4 Protein, Znfn1a4 Protein, EOS Protein, ZNFN1A4 Protein, IKAROS family zinc finger 4 Protein, Ikzf4 Protein, IKZF4 Protein

Background: Zinc finger protein Eos (Ikaros family zinc finger protein 4),FUNCTION: DNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems. Essential for the inhibitory function of regulatory T-cells (Treg). Mediates FOXP3-mediated gene silencing in regulatory T-cells (Treg) via recruitment of corepressor CTBP1 (PubMed:19696312). {ECO:0000269|PubMed:10218586, ECO:0000269|PubMed:17403896, ECO:0000269|PubMed:19696312}.

Molecular Weight: 64.2 kDa

UniProt: Q8C208