TCEB3 Protein (AA 1-773) (Strep Tag)

Catalog No. ABIN3136399

Product Details

Target: TCEB3 (Transcription Elongation Factor B (SIII), Polypeptide 3 (110kDa, Elongin A) (TCEB3))

Protein Type: Recombinant

Protein Characteristics: AA 1-773

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This TCEB3 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MAAESALQVV EKLQARLAAN PDPKKLLKYL KKLSILPITV DILVETGVGK TVNSFRKHEQ VGNFARDLVA QWKKLVPVER NSEAEDQDFE KNNSRKRPRD ALQREEELEG NYQESWKPSG SRSYSPEHRQ KKHKKLSEPE RPHKVAHSHE KRDERKRCHK VSPPYSSDPE SSDYGHVQSP PPSSPHQMYT DLSRSPEEDQ EPIISHQKPG KVHSNTFQDR LGVSHLGEQG KGAVSHHKQH RSSHKEKHPA DAREDEKISA VSREKSHKAS SKEESRRLLS GDSAKEKLPS SVVKKDKDRE GSSLKKKFSP ALDVASDNHF KKPKHKDSEK AKSDKNKQSV DGVDSGRGTG DPLPKAKEKV PNHLKAQEGK VRTNADGKSA GPLHPKAEET DVDDEFERPT MSFESYLSYD QPRKKKKKVV KTSSTALGEK GLKKKDSKST SKNLNSAQKL PKVNENKSEK LQPAGAEPTR PRKVPTDVLP ALPDIPLPAI HANYRPLPSL ELIPSFQPKR KAFSSPQEEE EAGFTGRRMN SKMQVYSGSK CAYLPKMMTL HQQCIRVLKN NIDSIFEVGG VPYSVLEPVL ERCTPDQLYR IEECNHVLIE ETDQLWKVHC HRDFKEERPE EYESWREMYL RLQDAREQRL RLLTNNIRSA HANKPKGRQA KMAFVNSVAK PPRDVRRRQE KFGTGGAAVP EKVRIKPAPY TTGSSHVPAS NSSSNFHSSP EELAYDGPST SSAHLAPVAS SSVSYDPRKP AVKKIAPMMA KTIKAFKNRF SRR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: TCEB3 (Transcription Elongation Factor B (SIII), Polypeptide 3 (110kDa, Elongin A) (TCEB3))

Alternative Name: Eloa (TCEB3 Products)

Synonyms: CG6755 Protein, Dmel\\CG6755 Protein, Elo-A Protein, dEloA Protein, tceb3a Protein, zgc:56638 Protein, wu:fw66g10 Protein, TCEB3 Protein, 110kDa Protein, AA408125 Protein, Tceb3a Protein, El Protein, EloA Protein, SIII Protein, SIII p110 Protein, TCEB3A Protein, Elongin A Protein, transcription elongation factor B polypeptide 3 Protein, elongin A Protein, calcium binding and coiled-coil domain 1b Protein, elongin A S homeolog Protein, transcription elongation factor B subunit 3 Protein, EloA Protein, LOC413407 Protein, eloa Protein, calcoco1b Protein, ELOA Protein, eloa.S Protein, Eloa Protein, TCEB3 Protein

Background: Elongin-A (EloA) (Elongin 110 kDa subunit) (RNA polymerase II transcription factor SIII subunit A1) (SIII p110) (Transcription elongation factor B polypeptide 3),FUNCTION: SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). {ECO:0000250|UniProtKB:Q63187}., FUNCTION: As part of a multisubunit complex composed of elongin BC complex (ELOB and ELOC), elongin A/ELOA, RBX1 and CUL5, polyubiquitinates monoubiquitinated POLR2A. {ECO:0000250|UniProtKB:Q14241}.

Molecular Weight: 87.2 kDa

UniProt: Q8CB77