RGS12 Protein (AA 1-1381) (Strep Tag)

Catalog No. ABIN3136449

Product Details

Target: RGS12 (Regulator of G-Protein Signalling 12 (RGS12))

Protein Type: Recombinant

Protein Characteristics: AA 1-1381

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This RGS12 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MYRAGEPGKR QPGPAPPRVR SVEVARGRAG YGFTLSGQAP CVLSCVMRGS PADFVGLRAG DQILAINEIN VKKASHEDVV KLIGKCSGVL HMVIAEGTSH VESCSSDEEG GLYEGKGWLR PKLDSKALGI NRAERVVEEV QSGGIFNMIF ESSSLCASGP EPLKLKQRSL SESAALRLDA GQAGLCAPHP SMLSKEDISK VINDDSVFTV GLDSHDDFGL DASILNVAMV VGYLGSIELP STSSNLEHDS LQAIRGCMRR LRAEQKIHSL VTMKVMHDCV QLVTDRAGVV AEYPAEKLAF SAVCPDDRRF FGLVTMQTND DGGLAQEDEG ALRTSCHVFM VDPDLFHHKI HQGIARRFGF ACTADPDTSG CLEFPASSLP VLQFISVLYR DMGELIEGVR ARAFLDGDAD AHQNNSTSSN SDSGIGNFNQ EEKSNRVLVV DLGGGSSRHG QGSSPGWESG GGRGSQPWSA PWNGAFCHDS EAGSPLETSP NTDRFWDLTK HSGPVSHMEV PPATLRSSIP PSKRGAAGSS CGFNQRWLPV HVLQEWQCGH ASDQESYTDS TDGWSSVNCG TLPPPMSKIP ADRYRVEGSF AQAPLSTQKR DWSRKAFGMQ NLFGPHRNVR KTKEDKKSSK LGRGVALAQT SQRTSARRSF GRSRRFSITR SLDDLESATV SDGELTGADL KDCISNNSLS SNASLPSVQS CRRLRERRVA SWAVSFERLL QDPVGVRYFS DFLRKEFSEE NILFWQACEC FSHVPAHDKK ELSYRAREIF SKFLCSKATT PVNIDSQAQL ADDILNAPHP DMFKEQQLQI FNLMKFDSYT RFLKSQLYQE CVLAEVEGRT LPDSQQVPSS PASKHSISSD HSNVSTPKKL SGKSKSGRSL NEDVGEEDSE KKRRGAFFSW SRSRSTGRSQ KKKDHGDHAH DAPHANGGLC RRESQGSVSS AGSLDLSEAC RTSALEKDKA AKHCCVHLPD GTSCVVAVKS GFSIKEILSG LCERHGINGA AVDLFLVGGD KPLVLHQDSS ILATRDLRLE KRTLFRLDLV PINRSVGLKA KPTKPVTEVL RPVVAKYGLD LGSLLVRLSG EKEPLDLGAP ISSLDGQRVI LEERDPSRGK VSTDKQKGAP VKQNSAVNSS PRNHLAMGEE RTLGKSNSIK IRGENGKSAR DPRLSKREES IAKIGKKKYQ KINLDEAEEF FELISKAQSN RADDQRGLLR KEDLVLPEFL RLPAGSSELA LSSPPPVKGY SKRAVTGHGQ EGAAQTEESY SDSPATSPAS AQSPCSAYSP GSAHSPGSAH STPGPPGTTQ PGEKPTKPSC VSMVQEGTTQ AWRRLSPEME AGGIQTVEDE QVADLTLMGE GDISSPNSTL LPPPPTPQDT PGPPRPGTSR F
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: RGS12 (Regulator of G-Protein Signalling 12 (RGS12))

Alternative Name: Rgs12 (RGS12 Products)

Synonyms: RGS12TS Protein, RGS12TS-I Protein, RGS12TS-L Protein, RGS12TS-S Protein, cb958 Protein, rgs12 Protein, 1200016K18Rik Protein, 4632412M04Rik Protein, AI481290 Protein, E130309H11 Protein, regulator of G protein signaling 12 Protein, regulator of G protein signaling 12b Protein, regulator of G-protein signaling 12 Protein, RGS12 Protein, rgs12b Protein, Rgs12 Protein

Background: Regulator of G-protein signaling 12 (RGS12),FUNCTION: Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. {ECO:0000250|UniProtKB:O08774}.

Molecular Weight: 149.6 kDa

UniProt: Q8CGE9

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling