DIS3L2 Protein (AA 1-870) (Strep Tag)

Catalog No. ABIN3136479

Product Details

Target: DIS3L2 (DIS3-Like Exonuclease 2 (DIS3L2))

Protein Type: Recombinant

Protein Characteristics: AA 1-870

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This DIS3L2 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MNHPDYKLNL RSPGTPRGVS SVVGPSAVGA SPGDKKSKNK SMRGKKKSIF ETYMSKEDVS EGLKRGTLIQ GVLRINPKKF HEAFIPSPDG DRDIFIDGVV ARNRALNGDL VVVKLLPEDQ WKAVKPESND KEIEATYEAD IPEEGCGHHP LQQSRKGWSG PDVIIEAQFD DSDSEDRHGN TSGLVDGVKK LSISTPDRGK EDSSTPVMKD ENTPIPQDTR GLSEKSLQKS AKVVYILEKK HSRAATGILK LLADKNSDLF KKYALFSPSD HRVPRIYVPL KDCPQDFMTR PKDFANTLFI CRIIDWKEDC NFALGQLAKS LGQAGEIEPE TEGILTEYGV DFSDFSSEVL ECLPQSLPWT IPPDEVGKRR DLRKDCIFTI DPSTARDLDD ALACRRLTDG TFEVGVHIAD VSYFVPEGSS LDKVAAERAT SVYLVQKVVP MLPRLLCEEL CSLNPMTDKL TFSVIWKLTP EGKILEEWFG RTIIRSCTKL SYDHAQSMIE NPTEKIPEEE LPPISPEHSV EEVHQAVLNL HSIAKQLRRQ RFVDGALRLD QLKLAFTLDH ETGLPQGCHI YEYRDSNKLV EEFMLLANMA VAHKIFRTFP EQALLRRHPP PQTKMLSDLV EFCDQMGLPM DVSSAGALNK SLTKTFGDDK YSLARKEVLT NMYSRPMQMA LYFCSGMLQD QEQFRHYALN VPLYTHFTSP IRRFADVIVH RLLAAALGYS EQPDVEPDTL QKQADHCNDR RMASKRVQEL SIGLFFAVLV KESGPLESEA MVMGVLNQAF DVLVLRFGVQ KRIYCNALAL RSYSFQKVGK KPELTLVWEP DDLEEEPTQQ VITIFSLVDV VLQAEATALK YSAILKRPGL EKASDEEPED
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: DIS3L2 (DIS3-Like Exonuclease 2 (DIS3L2))

Alternative Name: Dis3l2 (DIS3L2 Products)

Synonyms: RGD1560168 Protein, DIS3L2 Protein, DKFZp469O0522 Protein, FAM6A Protein, PRLMNS Protein, hDIS3L2 Protein, 4930429A22Rik Protein, 8030493P09Rik Protein, DIS3-like 3'-5' exoribonuclease 2 Protein, DIS3 like 3'-5' exoribonuclease 2 Protein, DIS3-like exonuclease 2 Protein, Dis3l2 Protein, DIS3L2 Protein, LOC100344728 Protein

Background: DIS3-like exonuclease 2 (EC 3.1.13.-),FUNCTION: 3'-5'-exoribonuclease that specifically recognizes RNAs polyuridylated at their 3' end and mediates their degradation. Component of an exosome-independent RNA degradation pathway that mediates degradation of both mRNAs and miRNAs that have been polyuridylated by a terminal uridylyltransferase, such as ZCCHC11/TUT4. Mediates degradation of cytoplasmic mRNAs that have been deadenylated and subsequently uridylated at their 3'. Mediates degradation of uridylated pre-let-7 miRNAs, contributing to the maintenance of embryonic stem (ES) cells. Essential for correct mitosis, and negatively regulates cell proliferation. {ECO:0000255|HAMAP-Rule:MF_03045, ECO:0000269|PubMed:23594738, ECO:0000269|PubMed:25119025}.

Molecular Weight: 97.8 kDa

UniProt: Q8CI75

Pathways: Stem Cell Maintenance