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SMC1B Protein (AA 1-1248) (His tag)

Crystallography grade SMC1B Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3136862
  • Target See all SMC1B Proteins
    SMC1B (Structural Maintenance of Chromosomes 1B (SMC1B))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-1248
    Origin
    Mouse
    Source
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This SMC1B protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    MGHLELLLVE NFKSWRGRQV IGPFKRFTCI IGPNGSGKSN VMDALSFVMG EKTTNLRVKN IQELIHGAHT GKPVSSSASV TIIYIEDSGE EKTFTRIIRG GCSEYHFGDK PVSRSVYVAQ LENIGIIVKA QNCLVFQGTV ESISMKKPKE RTQFFEEIST SGEFIGEYEA KKKKLQKAEE DAQFHFNVKK NVAAERKHAK IEKEEAEHYQ NLLEELKINK IQLMLFQLYY NEEKINVLNT ELEQMDGNLS VVKDTLSHHE NIFKAKKKDY GMLTRQLQQT AKELKSVEAI LNQKRPQYIK AKENTSHHLK KLDLSKKLIT DNEKQCSKQE DGIRALVAEL ADLDRAWKSF EKQMEEKILQ KGRDIELENS QLDRYKLLKE QVRRKVGIMT QQLEKLQWEQ KAEKERLAFE KRRHGDTQGN LKQIKEQIEE HKKRIEKLEE YTKTCMDCLE DKKQQEEALK KEIENTKSRM SEVNEELSLI RNELQNAGID NHEGKRQQKR AEVLEHLKRL YPDSVFGRLL DLCHPIHKKY QLAVTKLFGR YMVAIVVASE KIAKDCIRFL KAERAEPETF LALDYLDIKP INERLREIKG CKMMIDVIKT QFPQLKKVIQ FVCGNGLVCE TVEEARHIAF GGPERRKAVA LDGTLFLKSG VISGGSSDLK HKALCWDEKE LHNLRDKRSQ LVQELKELMK TLRKETDLKQ IQTLVQGTNT RLKYSQNELE MIKKKHLATF YREQSQLQSE LLNIDSQCTM LSEGINKQQQ KIEEFQDKID EVEDDIFQDF CEEIGVENIR EFENKHVKQQ QENDQKRLEF EKQKTRLNIQ LEYSRNQLKK KLNNIDTLKT TIQKGKEDID NLKKTEEECL KIVEELMVKQ EQIKEVLATQ SSNIEKIHIQ IEEERKKVLA VDREVGKLQK EVVIIQGSLE QKLLEKHNLL LDCKVQDIDI SLVLGSLEDI IEMELTETES TQATADIYEK EASIQIDYSP LREDLKALQS DKEVEAHLTL LLQQVASQEN TLLKTTAPNL RAQENLKTVR DKFQESADVF EASRKEARIC RQEFEQVKRR RYDAFSQCFE HISVSIDQIY KKLCRNNSAQ AFLSPENPEE PYLDGISYNC VAPGKRFMPM DNLSGGEKCV AALALLFAVH SFRPAPFFVL DEVDAALDNT NIGKVSSYIK EQSQEQFQMI IISLKEEFYS KADALIGVYP EHNECMFSHV LTLDLSKYPD TEDQEGSRSH RKPRVPRVSM SPKSPQSR
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Smc1b Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product SMC1B Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    SMC1B (Structural Maintenance of Chromosomes 1B (SMC1B))
    Alternative Name
    Smc1b (SMC1B Products)
    Synonyms
    SMC1BETA Protein, SMC1L2 Protein, bK268H5 Protein, bK268H5.5 Protein, SMC-1B Protein, SMC1beta Protein, Smc1l2 Protein, structural maintenance of chromosomes 1B Protein, SMC1B Protein, Smc1b Protein
    Background
    Meiosis-specific component of cohesin complex. Required for the maintenance of meiotic cohesion, but not, or only to a minor extent, for its establishment. Contributes to axial element (AE) formation and the organization of chromatin loops along the AE. Plays a key role in synapsis, recombination and chromosome movements. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The meiosis-specific cohesin complex probably replaces mitosis specific cohesin complex when it dissociates from chromatin during prophase I. {ECO:0000269|PubMed:11564881, ECO:0000269|PubMed:15146193}.
    Molecular Weight
    145.5 kDa Including tag.
    UniProt
    Q920F6
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