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IVNS1ABP Protein (AA 1-642) (His tag)

Crystallography grade IVNS1ABP Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3136864
  • Target See all IVNS1ABP Proteins
    IVNS1ABP (Influenza Virus NS1A Binding Protein (IVNS1ABP))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-642
    Origin
    Mouse
    Source
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This IVNS1ABP protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    MIPNGYLMFE DENFIESSVA KLNALRKSGQ FCDVRLQVCG HEMLAHRAVL ACCSPYLFEI FNSDSDPHGV SHVKLDDLNP EAVEVLLNYA YTAQLKADKE LVKDVYSAAK KLKMDRVKQV CGDYLLSRMD VTSCISYRNF ASCMGDSRLL NKVDAYIQEH LLQISEEEEF LKLPRLKLEV MLEDNVCLPS NGKLYTKVIN WVQRSIWENG DSLEELMEEV QTLYYSADHK LLDGNPLDGQ AEVFGSDDDH IQFVQKKPPR ENGHKQISGS STGCLSSPNA SMQSPKHEWK IVASEKTSNN TYLCLAVLDS TFCVIFLHGR NSPQSSPTST PKLSKSLSFE MQPDELLEKP MSPMQYARSG LGTAEMNGKL IAAGGYNREE CLRTVECYDP HTDHWSFLAP MRTPRARFQM AVLMGQLYVV GGSNGHSDDL SCGEMYDPNI DDWTPVPELR TNRCNAGVCA LNGKLYIVGG SDPYGQKGLK NCDVFDPVTK SWTSCAPLNI RRHQSAVCEL GGYLYIIGGA ESWNCLNTVE RYNPENNTWT LIAPMNVARR GAGVAVLDGK LFVGGGFDGS HAISCVEMYD PTRNEWKMMG NMTSPRSNAG ITTVGNTIYA VGGFDGNEFL NTVEVYNPQS NEWSPYTKIF QF
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Ivns1abp Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product IVNS1ABP Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    IVNS1ABP (Influenza Virus NS1A Binding Protein (IVNS1ABP))
    Alternative Name
    Ivns1abp (IVNS1ABP Products)
    Synonyms
    1190004M08Rik Protein, 1700126I16Rik Protein, AA960440 Protein, HSPC068 Protein, ND1 Protein, NS-1 Protein, NS1-BP Protein, Nd1-L Protein, Nd1-S Protein, mKIAA0850 Protein, cb1052 Protein, fj23g11 Protein, ivns1abp Protein, wu:fj23g11 Protein, FLARA3 Protein, KLHL39 Protein, NS1BP Protein, fi13f08 Protein, fi41c09 Protein, wu:fi13f08 Protein, wu:fi41c09 Protein, influenza virus NS1A binding protein Protein, influenza virus NS1A binding protein a Protein, influenza virus NS1A binding protein L homeolog Protein, influenza virus NS1A binding protein b Protein, Ivns1abp Protein, ivns1abpa Protein, ivns1abp.L Protein, IVNS1ABP Protein, ivns1abpb Protein
    Target Type
    Influenza Protein
    Background
    Plays a role in cell division and in the dynamic organization of the actin skeleton as a stabilizer of actin filaments by association with F-actin through Kelch repeats. Protects cells from cell death induced by actin destabilization, Protects neurons from dendritic spines and actin filaments damage induced by the actin-destabilizing cytochalasin B when overexpressed. Plays a role in protecting the heart from doxorubicin-induced cardiomyopathy. Activates Erk signaling pathway when overexpressed in cultured cell lines. {ECO:0000269|PubMed:11418182, ECO:0000269|PubMed:12213805, ECO:0000269|PubMed:15684717, ECO:0000269|PubMed:16317045, ECO:0000269|PubMed:16952015}.
    Molecular Weight
    72.5 kDa Including tag.
    UniProt
    Q920Q8
    Pathways
    Negative Regulation of intrinsic apoptotic Signaling
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