CDK5RAP3 Protein (AA 1-503) (Strep Tag)

Catalog No. ABIN3136958

Product Details

Target: CDK5RAP3 (CDK5 Regulatory Subunit Associated Protein 3 (CDK5RAP3))

Protein Type: Recombinant

Protein Characteristics: AA 1-503

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This CDK5RAP3 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MQDHQHVPID IQTSKLLDWL VDRRHCNLKW QSLVLTIREK INTAIQDMPE SQEIAQLLSG SYIHYFHCLR IVDLLKGTEA STKNIFGRYS SQRMKDWQEI VSLYEKDNTY LVELCSLLVR NVSYEIPSLK KQIAKCQQLQ QEYSRKEEEG QAGAAEMREQ FYHSCKQYGI TGDNVRRELL ALVKDLPSQL AEIGAGAQSL GEAIDLYQAC VEFVCDSPTE QVLPMLRYVQ KKGNSTVYEW RTGTEPSVVE RPQLEEPPEQ VQEDEIDWGD FGVEAVSDSG IVAETPGIDW GISLESEAKD AGADKIDWGD DAAAASEITV LETGTEAPEG VARGSDALTL LEYPETRNQF IDELMELEIF LSQRAVEMSE EADILSVSQF QLAPAILQGQ TKEKMLSLVS TLQQLIGRLT SLRMQHLFMI LASPRYVDRV TEFLQQKLKQ SQLLALKKEL MVEKQQEALQ EQAALEPKLD LLLEKTRELQ KLIEADISKR YSGRPVNLMG TSL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: CDK5RAP3 (CDK5 Regulatory Subunit Associated Protein 3 (CDK5RAP3))

Alternative Name: Cdk5rap3 (CDK5RAP3 Products)

Synonyms: cdkrap3 Protein, fb33h04 Protein, fc09c05 Protein, id:ibd2920 Protein, wu:fb33h04 Protein, wu:fc09c05 Protein, zgc:86863 Protein, CDK5RAP3 Protein, cdk5rap3 Protein, MGC82350 Protein, C53 Protein, HSF-27 Protein, IC53 Protein, LZAP Protein, MST016 Protein, OK/SW-cl.114 Protein, PP1553 Protein, 1810007E24Rik Protein, BC002318 Protein, C81486 Protein, CDK5 regulatory subunit associated protein 3 Protein, CDK5 regulatory subunit associated protein 3 L homeolog Protein, cdk5rap3 Protein, CDK5RAP3 Protein, cdk5rap3.L Protein, Cdk5rap3 Protein

Background: CDK5 regulatory subunit-associated protein 3,FUNCTION: Substrate adapter for ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to substrate proteins, in response to endoplasmic reticulum stress (PubMed:30635284). Negatively regulates NF-kappa-B-mediated gene transcription through the control of RELA phosphorylation (By similarity). Probable tumor suppressor initially identified as a CDK5R1 interactor controlling cell proliferation (By similarity). Also regulates mitotic G2/M transition checkpoint and mitotic G2 DNA damage checkpoint (By similarity). Through its interaction with CDKN2A/ARF and MDM2 may induce MDM2-dependent p53/TP53 ubiquitination, stabilization and activation in the nucleus, thereby promoting G1 cell cycle arrest and inhibition of cell proliferation (By similarity). May also play a role in the rupture of the nuclear envelope during apoptosis (By similarity). May regulate MAPK14 activity by regulating its dephosphorylation by PPM1D/WIP1 (By similarity). Required for liver development (PubMed:30635284). {ECO:0000250|UniProtKB:Q96JB5, ECO:0000269|PubMed:30635284}.

Molecular Weight: 57.0 kDa

UniProt: Q99LM2