RNF17 Protein (AA 1-1640) (Strep Tag)

Catalog No. ABIN3136980

Product Details

Target: RNF17 (Ring Finger Protein 17 (RNF17))

Protein Type: Recombinant

Protein Characteristics: AA 1-1640

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This RNF17 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MAAEASSTGL ASCHLVESKS GAQGASGCQC TRCGRKVSVA SGDHHKFPCG HAFCELCLLA PQEYTTSKCT DCEVHTTVSM NQGHYPVDGF IEEDSSLEAL PPKMVNNCSS DLEKTVDQLI NDLEHSSSIH RNVSNPSAVM SETEEIDEAL KIAGCNFEQL SNAIKMLDST QDQTRQETHS LTEAVEKQFD TLLASLDSRK KSLCEELIRR TDDYLSKLVT VKSYIEEKKS DLDAAMKIAK ELRSAPSLRT YCDLTQIIRT LKLTFESELS QVSSIIPRNT PRLDINCSEA ICMFSSMGKI EFEDSTKCYP QENEDGQNVQ KKFNNRKELC CDVYSSLEKK KVDAAVLTDE TPEPPLQAEA PDRHLEGKKK QPTKEMVVVT SPKTIAVLPQ LGSSPDVIIE EIIEENLESC FTDDPIETSG YPKKPPQKEQ SAPVGSKAGC PELVFVSHVI HPCHFYVRKY SQIKDATILE KKMKQVCNRS LHLDPSDILE LGARIFVNSI KNRMWCRGII TEIIPSKTKN IRKPCSPTKF SVCEISLIQI FMVDFGNSEV LIITGVGDTH EGPEHDGEQH ITLSDFCLLL MKSEPYSEEL LKDIPHLAHL CSLKDIVPYN STEGWEKEAK VEFLKMVNKK AVLMKVFGEE DDVLIVDLQK PPTNKISSDM PVSLRDALVF MELARFRSQS PRSHSEKNTT LCYHPPILPE EMTEVSVMVC HINSPTDFYL QLMENLDFLS LLKTIEEFYK GEDGENLEIL CPLQNQACVA KFEDGIWYRA KVIGLPGHRE VEVKYVDFGN TAKITLKDMR KIKDEFLEPP EKAIKCKLAY VEPSKKSQWS KKAKEKFEEK TQDKFVTCSV IKILENNVLL VELFDSRAPG KSAVSINDQL VKEGLASYEA GYTLKDNSKK HLEVWDPSPE EIITSEINNL SPLSVKSLPN ENFQSLYNKE LPVNICNVIS PEKIYVQWLL TENLLNSLEE KMVAAYEHSE WKPVKWECDM HCAVKVPAKN QWRRGQILRM VTDKLVEVLL YDVGVELVVN IHCLRELQEN LKTMGRLSLE CSLVDIRPTG GSDKWTATAC DCLSLHLTGA IATIILQESN TTWPLPVKIF CRDEKGERVD VSKYLIKKGL ALRERRVSKS SNSHSPEKSL EIPLEQGDSV VTKCFKINFD TNKKIADKVN EHKVPDSKGK KSESRSTGCY RPPAVPNTSS FEAIVTCIGD DGTIFVVPKL SEFELIKMMD EIQSNLKCLG LLEPYSWKKG EPCAVRGSDT LWYRGKVMEV VGGTIRVQYL DHGFTEKIPQ CHLYPILLYP DTPQFCIPCQ LYQTLPVGNT WQPDAIELLQ ELLSKREVDI HIMELPNNSW GKLSVHLYFD GMSLSHFMAH HKYCIFEHTE EIFKEKPRGQ NKKYEDENWK IRFEDLLLPE MEAPVLPPYL SSLLPPPEEL FAVQVKHIVS PDEMYICLDS EDSYTQFNHH GDTDDSGVSW ESESENLEEA LQRFNKNVET FPPLTDFSSE MPCLAEYADG LWYRAKIISI KEFNPLSVLV LFVDYGCTEK LTINRLRQIP VQLMQYPAQA IKVLLAGFKP PLSDSGKTRI PYCPKWSMEA LWTMIDCLQG KQLYASSVAQ APEQIVTLYE DEQYPVHMSL VEMGLADKDE
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: RNF17 (Ring Finger Protein 17 (RNF17))

Alternative Name: Rnf17 (RNF17 Products)

Synonyms: Mmip-2 Protein, SPATA23 Protein, TDRD4 Protein, MMIP-2 Protein, Mmip2 Protein, ring finger protein 17 Protein, RNF17 Protein, Rnf17 Protein

Background: RING finger protein 17 (Mad member-interacting protein 2) (Mmip-2),FUNCTION: Seems to be involved in regulation of transcriptional activity of MYC. In vitro, inhibits DNA-binding activity of Mad-MAX heterodimers. Can recruit Mad transcriptional repressors (MXD1, MXD3, MXD4 and MXI1) to the cytoplasm. May be involved in spermiogenesis. {ECO:0000269|PubMed:10597267, ECO:0000269|PubMed:11420703}.

Molecular Weight: 185.6 kDa

UniProt: Q99MV7