NUP155 Protein (AA 2-1391) (His tag)

Catalog No. ABIN3136998

Product Details

Target: NUP155 (Nucleoporin 155kDa (NUP155))

Protein Type: Recombinant

Protein Characteristics: AA 2-1391

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This NUP155 protein is labelled with His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA

Sequence: PSVLGSMMVA STSAAASLQE ALENAGRLID RQLQEDRMYP DLSELLMVSA PNSPTVSGMS DMDYPLQGPG LLSVPSLPEI STIRRVPLPP ELVEQFGHMQ CNCMMGVFPP ISRAWLTIDS DIFMWNYEDG GDLAYFDGLS ETILAVGLVK PKAGIFQPHV RHLLVLATPV DIVILGLSYA NVQTGSGILN DSMCGGMQLL PDPLYSLPTD NTYLLTITST DNGRIFLAGK DGCLYEVAYQ AEAGWFSQRC RKINHSKSSL SFLVPSLLQF TFSEDDPIVQ IEIDNSRNIL YTRSEKGVIQ VYDLGHDGQG MSRVASVSQN AIVSAAGNIA RTIDRSVFKP IVQIAVIESS ESLDCQLLAV THAGVRLYFS TCPFRQPLAR PNTLTLVHVR LPPGFSASST VEKPSKVHKA LYSKGILLMT ASENEDNDIL WCVNHDTFPF QKPMMETQMT TRVDGHSWAL SAIDELKVDK IITPLNKDHI PITDSPVVVQ QHMLPPKKFV LLSAQGSLMF HKLRPVDQLR HLLVSNVGGD GEEIERFFKL HQEDQACATC LILACSTAAC DREVSAWATR AFFRYGGEAQ MRFPATLPTP SNVGPILGSP MYSSSPVPSG SPYPNPSSLG TPSHGAQPPT MSTPMCAVGS PAMQAASMSG LTGPEIVYSG KHNGICIYFS RIMGNIWDAS LVVERVFKSS NREITAIESS VPVQLLESVL QELKGLQEFL DRNSQFSGGP LGNPNTTARV QQRLVGFMRP ENGNTQQMQQ ELQRKFQEAQ LSEKISLQAI QQLVRKSYQA LALWKLLCEH QFSVIVGELQ KEFQEQLKIT TFKDLVIRDK EVTGALIASL INCYIRDNAA VDGISLHLQD TCPLLYSTDD AVCSKANELL QRSRQVQSKT ERERMLRESL KEYQKISNQV DLPSVCAQYR QVRFYEGVVE LSLTAAEKKD PQGLGLHFYK HGEPEEDVVG LQTFQERLNS YKCITDTLQE LVNQSKAAPQ SPSVPKKPGP PVLSSDPNML SNEEAGHHFE QMLKLAQRSK DELFSIALYN WLIQADLADK LLQIASPFLE PHLVRMARVD QNRVRYMDLL WRYYEKNRSF SSAARVLSKL ADMHSTEISL QQRLEYIARA ILSAKSSTAI SSIAADGEFL HELEEKMEVA RIQLQIQETL QRQYSHHSSV QDAISQLDSE LMDITKLYGE FADPFKLAEC KLAVIHCAGY SDPILVHTLW QDIIEKELND SVALSSSDRM HALSLKLVLL GKIYAGTPRF FPLDFIVQFL EQQVCTLNWD VGFVIQTMNE IGVPLPRLLE VYDQLFKSRD PFWNRVKSPL HLLDCIHVLL TRYVENPSLV LNCERRRFTN LCLDAVCGYL VELQSMSSSV AVQAITGNFK SLQAKLERLH
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Nup155 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: NUP155 (Nucleoporin 155kDa (NUP155))

Alternative Name: Nup155 (NUP155 Products)

Synonyms: F10B6.25 Protein, F10B6_25 Protein, nucleoporin 155 Protein, DDBDRAFT_0189286 Protein, DDBDRAFT_0235243 Protein, DDB_0189286 Protein, DDB_0235243 Protein, D930027M19Rik Protein, mKIAA0791 Protein, zgc:55435 Protein, N155 Protein, nucleoporin 155 Protein, nucleoporin 155kDa Protein, nuclear pore protein Protein, nucleoporin 155kDa L homeolog Protein, NUP155 Protein, nup155 Protein, Nup155 Protein, nup155.L Protein

Background: Essential component of nuclear pore complex. Could be essessential for embryogenesis. Nucleoporins may be involved both in binding and translocating proteins during nucleocytoplasmic transport. {ECO:0000250|UniProtKB:P37199, ECO:0000269|PubMed:19070573}.

Molecular Weight: 155.9 kDa Including tag.

UniProt: Q99P88

Pathways: Protein targeting to Nucleus