CLCA1 Protein (AA 22-913) (rho-1D4 tag,His tag)

Catalog No. ABIN3137110

Product Details

Target: CLCA1 (Chloride Channel Accessory 1 (CLCA1))

Protein Type: Recombinant

Protein Characteristics: AA 22-913

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This CLCA1 protein is labelled with rho-1D4 tag,His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: MHHHHHHESL IQLNNNGYEG IVIAIDHDVP EDEALIQHIK DMVTQASPYL FEATGKRFYF KNVA ILI PESWKAKPEY TRPKLETFKN ADVLVSTTSP LGNDEPYTEH IGACGEKGIR IHLTPDFLAG KKLTQYGPQD RTFVHEWAHF RWGVFNEYNN DEKFYLSKGK PQAVRCSAAI TGKNQVRRCQ GGSCITNGKC VIDRVTGLYK DNCVFVPDPH QNEKASIMFN QNINSVVEFC TEKNHNQEAP NDQNQRCNLR STWEVIQESE DFKQTTPMTA QPPAPTFSLL QIGQRIVCLV LDKSGSMLND DRLNRMNQAS RLFLLQTVEQ GSWVGMVTFD SAAYVQSELK QLNSGADRDL LIKHLPTVSA GGTSICSGLR TAFTVIKKKY PTDGSEIVLL TDGEDNTISS CFDLVKQSGA IIHTVALGPA AAKELEQLSK MTGGLQTYSS DQVQNNGLVD AFAALSSGNA AIAQHSIQLE SRGVNLQNNQ WMNGSVIVDS SVGKDTLFLI TWTTHPPTIF IWDPSGVEQN GFILDTTTKV AYLQVPGTAK VGFWKYSIQA SSQTLTLTVT SRAASATLPP ITVTPVVNKN TGKFPSPVTV YASIRQGASP ILRASVTALI ESVNGKTVTL ELLDNGAGAD ATKNDGVYSR FFTAFDANGR YSVKIWALGG VTSDRQRAAP PKNRAMYIDG WIEDGEVRMN PPRPETSYVQ DKQLCFSRTS SGGSFVATNV PAAAPIPDLF PPCQITDLKA SIQGQNLVNL TWTAPGDDYD HGRASNYIIR MSTSIVDLRD HFNTSLQVNT TGLIPKEASS EEIFEFELGG NTFGNGTDIF IAIQAVDKSN LKSEISNIAR VSVFIPAQEP PIPEDSTPPC PDISINSTIP GIHVLKIMWK WLGEMQVTLG LHGSSGTETS QVAP A
Sequence includes N-terminal His-tag and C-terminal Rho1D4 tag

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PLP1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: The protein is purified from the cleared cell lysate using Rho1D4-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In solution (20 mM Hepes, pH 7.4, 100 mM NaCl, ortho-Phenanthroline, 5 mM EDTA

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: CLCA1 (Chloride Channel Accessory 1 (CLCA1))

Alternative Name: Clca1 (CLCA1 Products)

Synonyms: CACC Protein, CACC1 Protein, CLCRG1 Protein, CaCC-1 Protein, GOB5 Protein, hCLCA1 Protein, hCaCC-1 Protein, Clca3 Protein, fi39d11 Protein, wu:fi39d11 Protein, AECC Protein, Clca1 Protein, Clca2 Protein, gob-5 Protein, gob5 Protein, chloride channel accessory 1 Protein, chloride channel Protein, chloride channel protein Protein, CLCA1 Protein, Clca1 Protein, clca1 Protein, clcA1 Protein

Background: May be involved in mediating calcium-activated chloride conductance. May play critical roles in goblet cell metaplasia, mucus hypersecretion, cystic fibrosis and AHR. May be involved in the regulation of mucus production and/or secretion by goblet cells. Involved in the regulation of tissue inflammation in the innate immune response. May play a role as a tumor suppressor. Induces MUC5AC. {ECO:0000269|PubMed:11296262, ECO:0000269|PubMed:11694454, ECO:0000269|PubMed:16645179}.

Molecular Weight: 99.9 kDa including both tags

UniProt: Q9D7Z6