ALG13 Protein (AA 1-1166) (Strep Tag)

Catalog No. ABIN3137112

Product Details

Target: ALG13 (Asparagine-Linked Glycosylation 13 Homolog (ALG13))

Protein Type: Recombinant

Protein Characteristics: AA 1-1166

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This ALG13 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MKRAFVTVGT TSFDELVARV VANDCVQILE SLGYNHLVLQ VGRGTVVPKP FRTESFTLDV YRYKDSLKED LQQADLVISH AGAGSCLESL EKGKPLVVVV NEKLMNNHQF ELAKQLHKEG HLFYCTCRVL SCPAPVSLLL VLLGSAKILQ QLPSATLSCF GYLPTQAPVL VATAYSYLHS VFSSFPPLST FLIIPCTMQK GWKKYCGQKS LNEASMDEYL GSLGLFRKVV AKDASCLFRA ISEQLFHSQI HHLQIRRACV SYMKENQQAF ESYVEGSFEK YLERLGDPKE SAGQLELKAL SLIYNRDFII YRYPGKPPTQ VTDNGFEDKI ILCYSNNGHY DSVYSKEFQS TAGICQAILY ELLYKDVFVV DEETLKTAVD LFRSGSRRNK HHALTASVEG SSDQKSSTED RTEEAAACSS AASTPEGNKQ GTERQKVPES PSKMLFPYKV LKALDPEIYR NVEFDAWLDS RKELQKSECV EYGGRYYFLG DKCQVCMESG GKYYNAHIQE IDNDKSSVVV FIEEFAERHS IPLAHVRPVN QVALLPSWNA IPIRNGRGYP TITGGYFPEI VMTDMNMKQR KKMFKKFRGK EIYMTMAYSR GDPLVPSRIQ HSMHYGHDPL LYYSQTAGHI LSSQHFYPQH SSQRQGRGYG MPRDSSHLIS KQNLPNPKVG FCSGSGRKCC QSYDNVSYRS RSFRRSHRQM HCMNKGCQYG FAPENGVEET VTFYALEEGN ETAYSTLPNN GGPTTMVPAT SGYCVARQGY NSCKPPLNSG DSNDHCDNGG YHGDYLYSSE QGYETSSVYT TTVSTANLSL QDSGPSSVPQ DTVTSYNYPQ KVLENSAAIA VSWASHVPVP VIPNCAGDNE ALRTSDISSQ NAIQPGFVPP PAQGSPAYLE PSAAGAAGAA AAAAAAAAPV ATPVAAPLPL PPPLPPPPPA TLEAGDASGF PLPPPPPPPP PPPPPYSYDP SGSDLPQDTK VLQYYFNLGL QCYHHNYWHP MVYMPHVQQQ LQPQPQPQPQ PQPQPQPQPQ PQPQQPQQQQ PPPQQQQQQQ EQVHGESYPD CTEQPLVDQS APQVYSDVVR EDGTQADVST NDTFPIADAV PLPHGAVYYP VMTDPYGSPL LGFDSYVPVA SDYSSIAMWH PVNAACGASA QIHGAMNPGP IGYMLLPNSP HYTPQN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ALG13 (Asparagine-Linked Glycosylation 13 Homolog (ALG13))

Alternative Name: Alg13 (ALG13 Products)

Synonyms: 2810046O15Rik Protein, 4833435D08Rik Protein, Glt28d1 Protein, MDS031 Protein, OTTMUSG00000018917 Protein, CDG1S Protein, CXorf45 Protein, GLT28D1 Protein, TDRD13 Protein, YGL047W Protein, alg13 Protein, otul Protein, asparagine-linked glycosylation 13 Protein, ALG13, UDP-N-acetylglucosaminyltransferase subunit Protein, ALG13, UDP-N-acetylglucosaminyltransferase subunit L homeolog Protein, Alg13 Protein, ALG13 Protein, alg13.L Protein, alg13 Protein

Background: Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13 (EC 2.4.1.141) (EC 3.4.19.12) (Asparagine-linked glycosylation 13 homolog) (Glycosyltransferase 28 domain-containing protein 1) (UDP-N-acetylglucosamine transferase subunit ALG13 homolog),FUNCTION: [Isoform 1]: Possible multifunctional enzyme with both glycosyltransferase and deubiquitinase activities., FUNCTION: [Isoform 2]: May be involved in protein N-glycosylation, second step of the dolichol-linked oligosaccharide pathway (By. similarity).

Molecular Weight: 128.7 kDa

UniProt: Q9D8C3