PHF10 Protein (AA 1-497) (Strep Tag)

Catalog No. ABIN3137113

Product Details

Target: PHF10 (PHD Finger Protein 10 (PHF10))

Protein Type: Recombinant

Protein Characteristics: AA 1-497

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This PHF10 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MTAAGPGAAP SPGRCDSDPA SPGAQSPKDD NEDNSNDGTH PCKRRRMGSG DSSRSCETSS QDLSFSYYPA ENLIEYKWPP DETGEYYMLQ EQVSEYLGVT SFKRKYPDLE RRDLSHKEKL YLRELNVITE TQCTLGLTAL RSDEVIDLMI KEYPAKHAEY SVILQEKERQ RITDHYKEYS QMQQQSTQKV EASKVPEYIK KAAKKAAEFN SNLNRERMEE RRAYFDLQTH VIQVPQGKYK VLPTDRTKVS SYPVALIPGQ FQEYYKRYSP DELRYLPLNT ALYEPPLDPE LPALDSDGDS DDGEDGGGDE KRKNKGTSDS SSGNVSEGDS PPDSQEDTFH GRQKSKDKMA TPRKDGSKRS VLSKSAPGYK PKVIPNALCG ICLKGKESNK KGKAESLIHC SQCDNSGHPS CLDMTMELVS MIKTYPWQCM ECKTCIICGQ PHHEEEMMFC DVCDRGYHTF CVGLGAIPSG RWICDCCQRA PPTPRKVGRR GKNSKEG
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PHF10 (PHD Finger Protein 10 (PHF10))

Alternative Name: Phf10 (PHF10 Products)

Synonyms: BAF45A Protein, XAP135 Protein, 1810055P05Rik Protein, AV024533 Protein, zgc:66233 Protein, zgc:85788 Protein, MGC97742 Protein, phf10-b Protein, PHD finger protein 10 Protein, PHD finger protein 10 S homeolog Protein, PHD finger protein 10 L homeolog Protein, PHF10 Protein, Phf10 Protein, phf10 Protein, phf10.S Protein, CpipJ_CPIJ008394 Protein, phf10.L Protein

Background: PHD finger protein 10 (BRG1-associated factor 45a) (BAF45a),FUNCTION: Involved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. {ECO:0000269|PubMed:17640523}.

Molecular Weight: 55.8 kDa

UniProt: Q9D8M7