NDST3 Protein (AA 35-873) (His tag)

Catalog No. ABIN3137188

Product Details

Target: NDST3 (N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 3 (NDST3))

Protein Type: Recombinant

Protein Characteristics: AA 35-873

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This NDST3 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: SGYKQESEVS GRASEVDCGD LQHIPSRLME VRRTMISDAS RTDPTVLVFV ESQYSSLGQD IIMMLESIRF HYHTEIAPGK GDLPALTDNV KGKYVLIIYE NILKYINMDS WNRSLLDKYC IEYGVGIIGF HKTSEKNLQS FQFRGFPFSI SGNLAVKDCC INPHSPLLRV TKSSKLDRGS LPGTDWTVFQ INHSTYQPVI FAKVKTPENL SPPISKHAFY ATIIHDLGLH DGIQRVLFGN NLNFWLHKLI FIDAISFLSG KRLTLSLDRY ILVDIDDIFV GKEGTRMNTN DVKALLDTQN LLRTQITNFT FNLGFSGKFY HTGTEEEDEG DDCLLGSVDE FWWFPHMWSH MQPHLFHNES SLIEQMILNK KFALEHGIPT DMGYAVSPHH SGVYPVHVQL YEAWKKVWNI KITSTEEYPH LKPARYRRGF IHKNIMVLPR QTCGLFTHTI FYKEYPGGPR ELDKSIHGGE LFFTVVLNPI SIFMTHLSNY GNDRLGLYTF VNLANFVQTW TNLRLQTLPP AQLAHKYFEL FPDQKDPLWQ NPCDDKRHRD IWSKEKTCDR LPKFLVIGPQ KTGTTALCLF LIMHPSILSN SPSPKSFEEV QFFNRNNYHR GIDWYMDFFP VPSNVTTDFL FEKSANYFHS EDAPKRAASL VPKAKIITIL IDPSDRAYSW YQHQRSHEDP AALKFSFYEV ISAGPNAPWE LRTLQKRCLV PGWYANHIER WLVYFPPFQL LIIDGQHLRT TPATVMDEVQ KFLGVSPHYN YSEALTFDSH KGFWCQLLEE GKTKCLGKSK GRKYPPMDSD SRAFLSSYYR DHNVELSKLL HRLGQPLPSW LRQELQKVR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Ndst3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: NDST3 (N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 3 (NDST3))

Alternative Name: Ndst3 (NDST3 Products)

Synonyms: NDST3 Protein, HSST3 Protein, 4921531K01Rik Protein, 4930511P15Rik Protein, N-deacetylase and N-sulfotransferase 3 Protein, N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 Protein, NDST3 Protein, ndst3 Protein, LOC100484094 Protein, LOC100539813 Protein, Ndst3 Protein

Background: Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has high deacetylase activity but low sulfotransferase activity. {ECO:0000269|PubMed:11087757}.

Molecular Weight: 97.9 kDa Including tag.

UniProt: Q9EQH7

Pathways: Glycosaminoglycan Metabolic Process