RIMS2 Protein (AA 1-1530) (Strep Tag)

Catalog No. ABIN3137201

Product Details

Target: RIMS2 (Regulating Synaptic Membrane Exocytosis 2 (RIMS2))

Protein Type: Recombinant

Protein Characteristics: AA 1-1530

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This RIMS2 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MSAPLGPRGR PAPTPAASQP PPQPEMPDLS HLTEEERKII LAVMDRQKKE EEKEQSVLKI KEEHKAQPTQ WFPFSGITEL VNNVLQPQQK QPNEKEPQTK LHQQFEMYKE QVKKMGEESQ QQQEQKGDAP TCGICHKTKF ADGCGHNCSY CQTKFCARCG GRVSLRSNKV MWVCNLCRKQ QEILTKSGAW FYNSGSNTLQ QPDQKVPRGL RNEEAPQEKK AKLHEQPQFQ GAPGDLSVPA VEKGRAHGLT RQDTIKNGSG VKHQIASDMP SDRKRSPSVS RDQNRRYEQS EEREDYSQYV PSDGTMPRSP SDYADRRSQR EPQFYEEPGH LNYRDSNRRG HRHSKEYIVD DEDVESRDEY ERQRREEEYQ ARYRSDPNLA RYPVKPQPYE EQMRIHAEVS RARHERRHSD VSLANAELED SRISLLRMDR PSRQRSVSER RAAMENQRSY SMERTREAQG QSSYPQRTSN HSPPTPRRSP IPLDRPDMRR ADSLRKQHHL DPSSAVRKTK REKMETMLRN DSLSSDQSES VRPPPPRPHK SKKGGKMRQV SLSSSEEELA STPEYTSCDD VELESESVSE KGDSQKGKRK TSEQGVLSDS NTRSERQKKR MYYGGHSLEE DLEWSEPQIK DSGVDTCSST TLNEEHSHSD KHPVTWQPSK DGDRLIGRIL LNKRLKDGSV PRDSGAMLGL KVVGGKMTES GRLCAFITKV KKGSLADTVG HLRPGDEVLE WNGRLLQGAT FEEVYNIILE SKPEPQVELV VSRPIGDIPR IPDSTHAQLE SSSSSFESQK MDRPSISVTS PMSPGMLRDV PQFLSGQLSI KLWFDKVGHQ LIVTILGAKD LPSREDGRPR NPYVKIYFLP DRSDKNKRRT KTVKKTLEPK WNQTFIYSPV HRREFRERML EITLWDQARV REEESEFLGE ILIELETALL DDEPHWYKLQ THDVSSLPLP RPSPYLPRRQ LHGESPTRRL QRSKRISDSE VSDYDCEDGV GVVSDYRHNG RDLQSSTLSV PEQVMSSNHC SPSGSPHRVD VIGRTRSWSP SAPPPQRNVE QGHRGTRATG HYNTISRMDR HRVMDDHYSS DRDRSHPRTG SVQTSPSSTP GTGRRGRQLP QLPPKGTLER SAMDIEERNR QMKLNKYKQV AGSDPRLEQD YHSKYRSGWD PHRGADTVST KSSDSDVSDV SAVSRTSSAS RFSSTSYMSV QSERPRGNRK ISVFTSKMQN RQMGVSGKNL TKSTSISGDM CSLEKNDGSQ SDTAVGALGT SGKKRRSSIG AKMVAIVGLS RKSRSASQLS QTEGGGKKLR STVQRSTETG LAVEMRNWMT RQASRESTDG SMNSYSSEGN LIFPGVRLAS DSQFSDFLDG LGPAQLVGRQ TLATPAMGDI QVGMMDKKGQ LEVEIIRARG LVVKPGSKTL PAPYVKVYLL DNGVCIAKKK TKVARKTLEP LYQQLLSFEE SPQGRVLQII VWGDYGRMDH KSFMGVAQIL LDELELSNMV IGWFKLFPPS SLVDPTLAPL TRRASQSSLE SSTGPSYSRS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: RIMS2 (Regulating Synaptic Membrane Exocytosis 2 (RIMS2))

Alternative Name: Rims2 (RIMS2 Products)

Synonyms: OBOE Protein, RAB3IP3 Protein, RIM2 Protein, 2810036I15Rik Protein, AW048769 Protein, Rab3ip2 Protein, Rim2(+40A) Protein, Rim2(+44A) Protein, Rim2(+4A) Protein, Serg2 Protein, Syt3-rs Protein, mKIAA0751 Protein, Nim2 Protein, Rim2 Protein, RIMS2 Protein, MGC159469 Protein, rims2 Protein, zgc:73064 Protein, drs Protein, drsp Protein, mema Protein, np-a Protein, pinin Protein, pnn Protein, pnn-a Protein, pnn-b Protein, sdk3 Protein, regulating synaptic membrane exocytosis 2 Protein, regulating synaptic membrane exocytosis 2a Protein, pinin, desmosome associated protein S homeolog Protein, RIMS2 Protein, Rims2 Protein, rims2a Protein, pnn.S Protein

Background: Regulating synaptic membrane exocytosis protein 2 (Rab-3-interacting molecule 2) (RIM 2) (Rab-3-interacting protein 2),FUNCTION: Rab effector involved in exocytosis. May act as scaffold protein. Plays a role in dendrite formation by melanocytes (By similarity). {ECO:0000250|UniProtKB:Q9UQ26}.

Molecular Weight: 172.9 kDa

UniProt: Q9EQZ7

Pathways: Hormone Transport, Synaptic Vesicle Exocytosis