FLII Protein (AA 1-1271) (Strep Tag)

Catalog No. ABIN3137288

Product Details

Target: FLII (Flightless I Homolog (FLII))

Protein Type: Recombinant

Protein Characteristics: AA 1-1271

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This FLII protein is labelled with Strep Tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Sequence: MEATGVLPFV RGVDLSGNDF KGGYFPENVK AMTSLRWLKL NRTGLCYLPE ELAALQKLEH LSVSHNHLTT LHGELSSLPS LRAIVARANS LKNSGVPDDI FKLDDLSVLD LSHNQLTECP RELENAKNML VLNLSHNGID SIPNQLFINL TDLLYLDLSE NRLESLPPQM RRLVHLQTLV LNGNPLLHAQ LRQLPAMMAL QTLHLRNTQR TQSNLPTSLE GLSNLSDVDL SCNDLTRVPE CLYTLPSLRR LNLSSNQIAE LSLCIDQWVH LETLNLSRNQ LTSLPSAICK LTKLKKLYLN SNKLDFDGLP SGIGKLTSLE EFMAANNNLE LIPESLCRCP KLKKLVLNKN RLVTLPEAIH FLTEIQVLDV RENPSLVMPP KPADRTAEWY NIDFSLQNQL RLAGASPATV AAAAAVGSGS KDPLARKMRL RRRKDSAQDV QAKQVLKGMS DVAQEKNKNQ EESIDARAPG GKVRRWDQGL EKPRLDYSEF FTEDVGQLPG LTIWQIENFV PVLVEEAFHG KFYEADCYIV LKTFLDDSGS LNWEIYYWIG GEATLDKKAC SAIHAVNLRN YLGAECRTVR EEMGDESEEF LQVFDNDISY IEGGTASGFY TVEDTHYVTR MYRVYGKKNI KLEPVPLKGS SLDPRFVFLL DQGLDIYVWR GAQATLSNTT KARLFAEKIN KNERKGKAEI TLLVQGQEPP GFWDVLGGEP SEIKNHVPDD FWPPQPKLYK VGLGLGYLEL PQINYKLSVE HKKRPKVELM PGMRLLQSLL DTRCVYILDC WSDVFIWLGR KSPRLVRAAA LKLGQELCGM LHRPRHTVVS RSLEGTEAQV FKAKFKNWDD VLTVDYTRNA EAVLQGQGLS GKVKRDTEKT DQMKADLTAL FLPRQPPMPL AEAEQLMEEW NEDLDGMEGF VLEGRKFTRL PEEEFGHFYT QDCYVFLCRY WVPVEYEEEE KTEDKEGKAS AEAREGEEAA AEAEEKQPEE DFQCIVYFWQ GREASNMGWL TFTFSLQKKF ESLFPGKLEV VRMTQQQENP KFLSHFKRKF IIHRGKRKVT QGTLQPTLYQ IRTNGSALCT RCIQINTDSS LLNSEFCFIL KVPFESEDNQ GIVYAWVGRA SDPDEAKLAE DILNTMFDAS YSKQVINEGE EPENFFWVGI GAQKPYDDDA EYMKHTRLFR CSNEKGYFAV TEKCSDFCQD DLADDDIMLL DNGQEVYMWV GTQTSQVEIK LSLKACQVYI QHTRSKEHER PRRLRLVRKG NEQRAFTRCF HAWSTFRQAP A
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: FLII (Flightless I Homolog (FLII))

Alternative Name: Flii (FLII Products)

Synonyms: FLI Protein, FLIL Protein, Fli1 Protein, 3632430F08Rik Protein, Fliih Protein, im:7141769 Protein, DKFZp459O043 Protein, flightless Protein, FLII, actin remodeling protein Protein, flightless I actin binding protein Protein, protein flightless-1 homolog Protein, FLII, actin remodeling protein S homeolog Protein, Protein flightless-1 homolog Protein, FLII Protein, Flii Protein, flii Protein, LOC585336 Protein, LOC100640615 Protein, flii.S Protein, fli-1 Protein

Background: Protein flightless-1 homolog,FUNCTION: May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling (By similarity). Essential for early embryonic development. May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration, by inhibiting Rac1-dependent paxillin phosphorylation. {ECO:0000250, ECO:0000269|PubMed:11171324, ECO:0000269|PubMed:11971982, ECO:0000269|PubMed:21430700, ECO:0000269|PubMed:22581781}.

Molecular Weight: 144.8 kDa

UniProt: Q9JJ28