SYT7 Protein (AA 38-403) (His tag)

Catalog No. ABIN3137431

Product Details

Target: SYT7 (Synaptotagmin VII (SYT7))

Protein Type: Recombinant

Protein Characteristics: AA 38-403

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This SYT7 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: CHWCQRKLGK RYKNSLETVG TPDSGRGRGE KKAIKLPAGG KAVNTAPVPG QTPHDESDRR TETRSSVSDL VNSLTSEMLM LSPGSEEDEA HEGCSRENLG RIQFSVGYNF QESTLTVKVM KAQELPAKDF SGTSDPFVKI YLLPDKKHKL ETKVKRKNLN PHWNETFLFE GFPYEKVVQR VLYLQVLDYD RFSRNDPIGE VSIPLNKVDL TQMQTFWKDL KPCSDGSGSR GELLLSLCYN PSANSIIVNI IKARNLKAMD IGGTSDPYVK VWLMYKDKRV EKKKTVTKKR NLNPIFNESF AFDIPTEKLR ETTIIITVMD KDKLSRNDVI GKIYLSWKSG PGEVKHWKDM IARPRQPVAQ WHQLKA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Syt7 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SYT7 (Synaptotagmin VII (SYT7))

Alternative Name: Syt7 (SYT7 Products)

Synonyms: CG10322 Protein, CG2381 Protein, Dmel\\CG2381 Protein, SYT7 Protein, Syt 7 Protein, Syt VII Protein, dsyt7 Protein, syt7 Protein, MGC145945 Protein, IPCA-7 Protein, IPCA7 Protein, PCANAP7 Protein, SYT-VII Protein, SYTVII Protein, AI851541 Protein, B230112P13Rik Protein, Synaptotagmin 7 Protein, synaptotagmin VII Protein, synaptotagmin 7 Protein, Syt7 Protein, SYT7 Protein, syt7 Protein

Background: Ca(2+) sensor involved in Ca(2+)-dependent exocytosis of secretory and synaptic vesicles through Ca(2+) and phospholipid binding to the C2 domain. Ca(2+) induces binding of the C2-domains to phospholipid membranes and to assembled SNARE-complexes, both actions contribute to triggering exocytosis. SYT7 binds Ca(2+) with high affinity and slow kinetics compared to other synaptotagmins (PubMed:26738595). Involved in Ca(2+)-triggered lysosomal exocytosis, a major component of the plasma membrane repair (By similarity). Ca(2+)-regulated delivery of lysosomal membranes to the cell surface is also involved in the phagocytic uptake of particles by macrophages (PubMed:16982801, PubMed:21041449). Ca(2+)-triggered lysosomal exocytosis also plays a role in bone remodeling by regulating secretory pathways in osteoclasts and osteoblasts (PubMed:18539119). Involved in cholesterol transport from lysosome to peroxisome by promoting membrane contacts between lysosomes and peroxisomes: probably acts by promoting vesicle fusion by binding phosphatidylinositol-4,5-bisphosphate on peroxisomal membranes (PubMed:25860611). Acts as a key mediator of synaptic facilitation, a process also named short-term synaptic potentiation: synaptic facilitation takes place at synapses with a low initial release probability and is caused by influx of Ca(2+) into the axon terminal after spike generation, increasing the release probability of neurotransmitters (PubMed:24569478, PubMed:26738595). Probably mediates synaptic facilitation by directly increasing the probability of release (PubMed:26738595). May also contribute to synaptic facilitation by regulating synaptic vesicle replenishment, a process required to ensure that synaptic vesicles are ready for the arrival of the next action potential: SYT7 is required for synaptic vesicle replenishment by acting as a sensor for Ca(2+) and by forming a complex with calmodulin (PubMed:24569478). Also acts as a regulator of Ca(2+)-dependent insulin and glucagon secretion in beta-cells (PubMed:18308938, PubMed:19171650). Triggers exocytosis by promoting fusion pore opening and fusion pore expansion in chromaffin cells (PubMed:20956309). Also regulates the secretion of some non-synaptic secretory granules of specialized cells (By similarity). {ECO:0000250|UniProtKB:Q62747, ECO:0000269|PubMed:16982801, ECO:0000269|PubMed:18308938, ECO:0000269|PubMed:18539119, ECO:0000269|PubMed:19171650, ECO:0000269|PubMed:20956309, ECO:0000269|PubMed:21041449, ECO:0000269|PubMed:24569478, ECO:0000269|PubMed:25860611, ECO:0000269|PubMed:26738595}.

Molecular Weight: 42.6 kDa Including tag.

UniProt: Q9R0N7

Pathways: Synaptic Vesicle Exocytosis