MAGI2 Protein (AA 1-1275) (Strep Tag)

Catalog No. ABIN3137553

Product Details

Target: MAGI2 (Membrane Associated Guanylate Kinase, WW and PDZ Domain Containing 2 (MAGI2))

Protein Type: Recombinant

Protein Characteristics: AA 1-1275

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This MAGI2 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MSKSLKKKSH WTSKVHESVI GRNPEGQLGF ELKGGAENGQ FPYLGEVKPG KVAYESGSKL VSEELLLEVN ETPVAGLTIR DVLAVIKHCK DPLRLKCVKQ GGIVDKDLRH YLNLRFQKGS VDHELQQIIR DNLYLRTVPC TTRPHKEGEV PGVDYIFITV EEFMELEKSG ALLESGTYED NYYGTPKPPA EPAPLLNVTD QILPGATPSA EGKRKRNKSV TNMEKASIEP PEEEEEERPV VNGNGVVITP ESSEHEDKSA GASGETPSQP YPAPVYSQPE ELKDQMDDTK PTKPEENEDS DPLPDNWEMA YTEKGEVYFI DHNTKTTSWL DPRLAKKAKP PEECKENELP YGWEKIDDPI YGTYYVDHIN RRTQFENPVL EAKRKLQQHN MPHTELGAKP LQAPGFREKP LFTRDASQLK GTFLSTTLKK SNMGFGFTII GGDEPDEFLQ VKSVIPDGPA AQDGKMETGD VIVYINEVCV LGHTHADVVK LFQSVPIGQS VNLVLCRGYP LPFDPEDPAN SMVPPLAIME RPPPVMVNGR HNYETYLEYI SRTSQSVPDI TDRPPHSLHS MPADGQLDGT YPPPVHDDNV SMASSGATQA ELMTLTIVKG AQGFGFTIAD SPTGQRVKQI LDIQGCPGLC EGDLIVEINQ QNVQNLSHTE VVDILKDCPV GSETSLIIHR GGFFSPWKTP KPMMDRWENQ GSPQTSLSAP AVPQNLPFPP ALHRSSFPDS TEAFDPRKPD PYELYEKSRA IYESRQQVPP RTSFRMDSSG PDYKELDVHL RRMESGFGFR ILGGDEPGQP ILIGAVIAMG SADRDGRLHP GDELVYVDGI PVAGKTHRYV IDLMHHAARN GQVNLTVRRK VLCGGEPCPE NGRSPGSVST HHSSPRSDYA TYSNSNHAAP SSNASPPEGF ASHSLQTSDV VIHRKENEGF GFVIISSLNR PESGATITVP HKIGRIIDGS PADRCAKLKV GDRILAVNGQ SIINMPHADI VKLIKDAGLS VTLRIIPQEE LNSPTSAPSS EKQSPMAQQH SPLAQQSPLA QPSPATPNSP VAQPAPPQPL QLQGHENSYR SEVKARQDVK PDIRQPPFTD YRQPPLDYRQ PPGGDYSQPP PLDYRQHSPD TRQYPLSDYR QPQDFDYFTV DMEKGAKGFG FSIRGGREYK MDLYVLRLAE DGPAIRNGRM RVGDQIIEIN GESTRDMTHA RAIELIKSGG RRVRLLLKRG TGQVPEYGMV PSSLSMCMKS DKHGSPYFYL LGHPKDTTNP TPGVLPLPPP QACRK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: MAGI2 (Membrane Associated Guanylate Kinase, WW and PDZ Domain Containing 2 (MAGI2))

Alternative Name: Magi2 (MAGI2 Products)

Synonyms: MAGI2 Protein, si:dkey-97c7.1 Protein, si:dkeyp-86a5.1 Protein, ACVRIP1 Protein, AIP-1 Protein, AIP1 Protein, ARIP1 Protein, MAGI-2 Protein, SSCAM Protein, Acvri1 Protein, Acvrinp1 Protein, Acvrip1 Protein, Magi-2 Protein, S-SCAM Protein, mKIAA0705 Protein, membrane associated guanylate kinase, WW and PDZ domain containing 2 Protein, membrane associated guanylate kinase, WW and PDZ domain containing 2a Protein, MAGI2 Protein, magi2a Protein, magi2 Protein, Magi2 Protein

Background: Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 (Activin receptor-interacting protein 1) (Acvrip1) (Atrophin-1-interacting protein 1) (AIP-1) (Membrane-associated guanylate kinase inverted 2) (MAGI-2),FUNCTION: Seems to act as a scaffold molecule at synaptic junctions by assembling neurotransmitter receptors and cell adhesion proteins (By similarity). Plays a role in nerve growth factor (NGF)-induced recruitment of RAPGEF2 to late endosomes and neurite outgrowth (By similarity). May play a role in regulating activin-mediated signaling in neuronal cells (PubMed:10681527). Enhances the ability of PTEN to suppress AKT1 activation (By similarity). Plays a role in receptor-mediated clathrin-dependent endocytosis which is required for ciliogenesis (PubMed:24608321). {ECO:0000250|UniProtKB:O88382, ECO:0000250|UniProtKB:Q86UL8, ECO:0000269|PubMed:10681527, ECO:0000269|PubMed:24608321}.

Molecular Weight: 140.9 kDa

UniProt: Q9WVQ1

Pathways: Neurotrophin Signaling Pathway