EIF2S3X Protein (AA 2-472) (His tag)
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- Target
- EIF2S3X (Eukaryotic Translation Initiation Factor 2, Subunit 3, Structural Gene X-Linked (EIF2S3X))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-472
- Origin
- Mouse
- Source
- Insect Cells
- Purification tag / Conjugate
- This EIF2S3X protein is labelled with His tag.
- Application
- Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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AGGEGGVTLG QPHLSRQDLA TLDVTKLTPL SHEVISRQAT INIGTIGHVA HGKSTVVKAI SGVHTVRFKN ELERNITIKL GYANAKIYKL DDPSCPRPEC YRSCGSSTPD EFPTDIPGTK GNFKLVRHVS FVDCPGHDIL MATMLNGAAV MDAALLLIAG NESCPQPQTS EHLAAIEIMK LKHILILQNK IDLVKESQAK EQYEQILAFV QGTVAEGAPI IPISAQLKYN IEVVCEYIVK KIPVPPRDFT SEPRLIVIRS FDVNKPGCEV DDLKGGVAGG SILKGVLKVG QEIEVRPGIV SKDSEGKLMC KPIFSKIVSL FAEHNDLQYA APGGLIGVGT KIDPTLCRAD RMVGQVLGAV GALPEIFTEL EISYFLLRRL LGVRTEGDKK AAKVQKLSKN EVLMVNIGSL STGGRVSAVK ADLGKIVLTN PVCTEVGEKI ALSRRVEKHW RLIGWGQIRR GVTIKPTVDD D
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Eif2s3x Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- EIF2S3X (Eukaryotic Translation Initiation Factor 2, Subunit 3, Structural Gene X-Linked (EIF2S3X))
- Alternative Name
- Eif2s3x
- Synonyms
- AA409828 Protein, AA547477 Protein, AI314668 Protein, Eif-2gx Protein, eukaryotic translation initiation factor 2, subunit 3, structural gene X-linked Protein, Eif2s3x Protein
- Background
- As a subunit of eukaryotic initiation factor 2 (eIF2), involved in the early steps of protein synthesis. In the presence of GTP, eIF2 forms a ternary complex with initiator tRNA Met-tRNAi and then recruits the 40S ribosomal complex, a step that determines the rate of protein translation. This step is followed by mRNA binding to form the 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B (By similarity). Along with its paralog on chromosome Y, may contribute to spermatogenesis up to the round spermatid stage (PubMed:26823431). {ECO:0000250, ECO:0000269|PubMed:26823431}.
- Molecular Weight
- 51.9 kDa Including tag.
- UniProt
- Q9Z0N1
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