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BAF53A Protein (AA 2-429) (His tag)

Crystallography grade BAF53A Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3137639
  • Target See all BAF53A Proteins
    BAF53A (Actin-Like 6A (BAF53A))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 2-429
    Origin
    • 7
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Source
    • 6
    • 3
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This BAF53A protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    SGGVYGGDEV GALVFDIGSY TVRAGYAGED CPKVDFPTAI GVVLERDDGS TMMEIDGDKG KQGGPTYYID TNALRVPREN MEAISPLKNG MVEDWDSFQA ILDHTYKMHV KSEASLHPVL MSEAPWNTRA KREKLTELMF EHYSIPAFFL CKTAVLTAFA NGRSTGLILD SGATHTTAIP VHDGYVLQQG IVKSPLAGDF ITMQCRELFQ EMNIELIPPY MIASKEAVRE GSPANWKRKE KLPQVTRSWH NYMCNCVIQD FQASVLQVSD STYDEQVAAQ MPTVHYEFPN GYNCDFGAER LKIPEGLFDP SNVKGLSGNT MLGVSHVVTT SVGMCDIDIR PGLYGSVIVA GGNTLIQSFT DRLNRELSQK TPPSMRLKLI ANNTTVERRF SSWIGGSILA SLGTFQQMWI SKQEYEEGGK QCVERKCP
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Actl6a Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product BAF53A Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    BAF53A (Actin-Like 6A (BAF53A))
    Alternative Name
    Actl6a (BAF53A Products)
    Synonyms
    ACTL6 Protein, ARPN-BETA Protein, Arp4 Protein, BAF53A Protein, INO80K Protein, 2810432C06Rik Protein, AI851094 Protein, ARP4 Protein, Actl6 Protein, Baf53a Protein, C79802 Protein, baf53a Protein, fb52e06 Protein, fl26e12 Protein, chunp6900 Protein, wu:fb52e06 Protein, wu:fl26e12 Protein, actl6 Protein, MGC75671 Protein, RGD1307747 Protein, ACTL6A Protein, ATARP4 Protein, F15H18.8 Protein, F15H18_8 Protein, actin-related protein 4 Protein, Actin-like protein 4 Protein, CaO19.5623 Protein, actin Protein, actl6a Protein, actl6a.L Protein, arp4 Protein, arpn-beta Protein, ino80k Protein, actin like 6A Protein, actin-like 6A Protein, actin-related protein 4 Protein, Arp4p Protein, actin like 6A S homeolog Protein, ACTL6A Protein, Actl6a Protein, actl6a Protein, ARP4 Protein, actl6a.S Protein
    Background
    Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for maximal ATPase activity of SMARCA4/BRG1/BAF190A and for association of the SMARCA4/BRG1/BAF190A containing remodeling complex BAF with chromatin/nuclear matrix. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair (By similarity). {ECO:0000250, ECO:0000269|PubMed:17640523}.
    Molecular Weight
    48.3 kDa Including tag.
    UniProt
    Q9Z2N8
    Pathways
    Chromatin Binding, Photoperiodism
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