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SART1 Protein (AA 1-806) (His tag)

Crystallography grade SART1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3137651
  • Target See all SART1 Proteins
    SART1 (Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-806
    Origin
    Mouse
    Source
    • 2
    • 1
    Insect Cells
    Purification tag / Conjugate
    This SART1 protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    MGSSKKHRGE KEAAGTTAAA GTGGTTEQPP RHREHKKHKH RSSGGGSSGG ERRKRSRERG SERGSGRRGA EAEARSGAHG RERSQAEPSE RRVKREKRDD GYEAAASSKA SSGDASSLSI EETNKLRAKL GLKPLEVNAV KKEAGTKEEP VAADVINPMA LRQREELREK LAAAKEKRLL NQKLGKIKTL GEDDPWLDDT AAWIERSRQL QKEKDLAEKR AKLLEEMDQE FGVSTLVEEE FEQRRQDLYS ARDLQGLTVE HAIDSFREGE TVVLTLKDKG VLQDGEDVLV NVNMVDKERA DKNVELRKKK PDYLPYAEDE SVDDLAQQKP RSILAKYDEE LEGERPHSFR LEQGGMADGL RERELEEIRT KLRLQAQSLS SVGPRLASEY LSPEEMVTFK KTKRRVKKIR KKEKEVIMRA DDLLPLGDQT QDGDFGSRLR GRGRRRVPEV EEEALEDEEK DPVAQPPPSD DTRVENMDIS DEEDGGALPP GSPEGLEEDE AELELQKQLE KGRRLRQLQQ LQQLRDSGEK VLEIVKKLES RQRGWEEEED PERKGTIVFN ATSEFCRTLG EIPTYGLAGN REEQEELMDF ERDEERSANG GSESDGEENI GWSTVNLDEE KQHQDFSASS TTILDEEPIV NRGLAAALLL CQNKGLLETT VQKVARVKAP NKSLPSAVYC IEDKMAIDDK YSRREEYRGF TQDFKEKDGY KPDVKIEYVD ETGRKLTPKE AFRQLSHRFH GKGSGKMKTE RRMKKLDEEA LLKKMSSSDT PLGTVALLQE KQKAQKTPYI VLSGSGKSMN ANTITK
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Sart1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product SART1 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    SART1 (Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1))
    Alternative Name
    Sart1 (SART1 Products)
    Synonyms
    Dmel\\CG6686 Protein, SART1 Protein, MGC53679 Protein, MGC132129 Protein, zgc:91927 Protein, Ara1 Protein, HOMS1 Protein, SART1259 Protein, SNRNP110 Protein, Snu66 Protein, U5-110K Protein, CG6686 gene product from transcript CG6686-RB Protein, SART1, U4/U6.U5 tri-snRNP-associated protein 1 S homeolog Protein, SART1, U4/U6.U5 tri-snRNP-associated protein 1 Protein, squamous-cell carcinoma T-cell-recognized antigen Protein, squamous cell carcinoma antigen recognized by T cells 1 Protein, CG6686 Protein, sart1.S Protein, sart1 Protein, SART1 Protein, Sart1 Protein
    Background
    Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA. Appears to play a role in hypoxia-induced regulation of EPO gene expression. {ECO:0000269|PubMed:10887110}.
    Molecular Weight
    91.8 kDa Including tag.
    UniProt
    Q9Z315
    Pathways
    Ribonucleoprotein Complex Subunit Organization
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