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Corin Protein (AA 134-1113) (His tag)

Crystallography grade CORIN Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Catalog No. ABIN3137654
  • Target See all Corin (CORIN) Proteins
    Corin (CORIN) (Corin, Serine Peptidase (CORIN))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 134-1113
    Origin
    • 12
    • 6
    • 2
    Mouse
    Source
    • 10
    • 8
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This Corin protein is labelled with His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequence
    SFVGTLKRVY FKSNDSEPLV TDGEARVPGV IPVNTVYYEN TGAPSLPPSQ STPAWTPRAP SPEDQSHRNT STCMNITHSQ CQILPYHSTL APLLPIVKNM DMEKFLKFFT YLHRLSCYQH ILLFGCSLAF PECVVDGDDR HGLLPCRSFC EAAKEGCESV LGMVNSSWPD SLRCSQFRDH TETNSSVRKS CFSLQQEHGK QSLCGGGESF LCTSGLCVPK KLQCNGYNDC DDWSDEAHCN CSKDLFHCGT GKCLHYSLLC DGYDDCGDLS DEQNCDCNLT KEHRCGDGRC IAAEWVCDGD HDCVDKSDEV NCSCHSQGLV ECRSGQCIPS TFQCDGDEDC KDGSDEENCS DSQTPCPEGE QGCLGSSCVE SCAGSSLCDS DSSLSNCSQC EPITLELCMN LPYNHTHYPN YLGHRTQKEA SISWESSLFP ALVQTNCYKY LMFFACTILV PKCDVNTGQR IPPCRLLCEH SKERCESVLG IVGLQWPEDT DCNQFPEESS DNQTCLLPNE DVEECSPSHF KCRSGRCVLG SRRCDGQADC DDDSDEENCG CKERALWECP FNKQCLKHTL ICDGFPDCPD SMDEKNCSFC QDNELECANH ECVPRDLWCD GWVDCSDSSD EWGCVTLSKN GNSSSLLTVH KSAKEHHVCA DGWRETLSQL ACKQMGLGEP SVTKLIPGQE GQQWLRLYPN WENLNGSTLQ ELLVYRHSCP SRSEISLLCS KQDCGRRPAA RMNKRILGGR TSRPGRWPWQ CSLQSEPSGH ICGCVLIAKK WVLTVAHCFE GREDADVWKV VFGINNLDHP SGFMQTRFVK TILLHPRYSR AVVDYDISVV ELSDDINETS YVRPVCLPSP EEYLEPDTYC YITGWGHMGN KMPFKLQEGE VRIIPLEQCQ SYFDMKTITN RMICAGYESG TVDSCMGDSG GPLVCERPGG QWTLFGLTSW GSVCFSKVLG PGVYSNVSYF VGWIERQIYI QTFLQKKSQG
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Corin Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product CORIN Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    Corin (CORIN) (Corin, Serine Peptidase (CORIN))
    Alternative Name
    Corin (CORIN Products)
    Synonyms
    CORIN Protein, CG2105 Protein, Dmel\\CG2105 Protein, SP75 Protein, LOC559109 Protein, AV273130 Protein, Lrp4 Protein, ATC2 Protein, CRN Protein, PEE5 Protein, TMPRSS10 Protein, corin, serine peptidase Protein, CG2105 gene product from transcript CG2105-RC Protein, novel protein similar to H.sapiens CORIN, corin, serine peptidase (CORIN) Protein, corin Protein, CORIN Protein, Corin Protein, LOC559109 Protein
    Background
    Serine-type endopeptidase involved in atrial natriuretic peptide hormone (NPPA) processing. Converts through proteolytic cleavage the non-functional propeptide NPPA into the active hormone, thereby regulating blood pressure in heart and promoting natriuresis, diuresis and vasodilation. Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Also acts as a regulator of sodium reabsorption in kidney. May also process pro-NPPB the B-type natriuretic peptide. {ECO:0000269|PubMed:11884416, ECO:0000269|PubMed:15637153, ECO:0000269|PubMed:20613715, ECO:0000269|PubMed:22418978, ECO:0000269|PubMed:22437503}.
    Molecular Weight
    110.2 kDa Including tag.
    UniProt
    Q9Z319
    Pathways
    Regulation of Systemic Arterial Blood Pressure by Hormones
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