CSF3R Protein (AA 25-621) (His tag,AVI tag,Biotin)

Catalog No. ABIN3137667

Product Details

Target: CSF3R (Colony Stimulating Factor 3 Receptor (Granulocyte) (CSF3R))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 25-621

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CSF3R protein is labelled with His tag,AVI tag,Biotin.

Brand: MABSol®,PrecisionAvi

Sequence: AA 25-621

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Characteristics: This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 69.2 kDa. The protein migrates as 72-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: CSF3R (Colony Stimulating Factor 3 Receptor (Granulocyte) (CSF3R))

Alternative Name: G-CSFR (CSF3R Products)

Synonyms: CD114 Protein, GCSFR Protein, Cd114 Protein, Csfgr Protein, G-CSF-R Protein, G-CSFR Protein, CSF3R Protein, gcsfr Protein, cd114 Protein, colony stimulating factor 3 receptor Protein, colony stimulating factor 3 receptor (granulocyte) Protein, colony stimulating factor 3 receptor L homeolog Protein, granulocyte colony-stimulating factor receptor Protein, CSF3R Protein, Csf3r Protein, csf3r Protein, csf3r.L Protein, LOC100433338 Protein

Background: The granulocyte colony-stimulating factor receptor (G-CSF-R) also known as CD114 (Cluster of Differentiation 114) is a protein that is encoded by the CSF3R gene. G-CSF-R is a cell-surface receptor for the granulocyte colony-stimulating factor (G-CSF). The G-CSF receptors belongs to a family of cytokine receptors known as the hematopoietin receptor family. Granulocyte colony-stimulating factor receptor has been shown to interact with Grb2, HCK and SHC1. The granulocyte colony-stimulating factor receptor is present on precursor cells in the bone marrow, and, in response to stimulation by G-CSF, initiates cell proliferation and differentiation into mature neutrophilic granulocytes and macrophages.

Molecular Weight: 69.2 kDa

NCBI Accession: NP_000751