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FUT10 Protein (AA 1-481) (rho-1D4 tag)

Crystallography grade FUT10 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3122460
  • Target See all FUT10 Proteins
    FUT10 (Fucosyltransferase 10 (Alpha (1,3) Fucosyltransferase) (FUT10))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-481
    Origin
    • 4
    • 1
    • 1
    • 1
    • 1
    Mouse
    Source
    • 3
    • 3
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This FUT10 protein is labelled with rho-1D4 tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    MVRFQRRKLL ASCLCVTATV FLMVTLQVVV ELGKFERKKL KDSNVQDGHR DVEGEPKHLE PFPEKEALAL AGRTKVDAGS YPIVLWWSPL TGETGRLGQC GADACFFTIN RTFQHHPMTR AFLFYGTDFN IDSLPLPREA HHDWALFHEE SPKNNYKLFH KPVITLFNHT ATFSRHSHLP LTTQYLEGVD VLKSLRYLVP LQAKNNLRQK LAPLVYVQSD CDPPSDRDSY VRELMAYIEV DSYGECLQNR DLPQQLKNPA SMDADAFYRV IAQYKFILAF ENAVCDDYIT EKFWRPLKLG VVPVYYGSPT IADWLPSNRS AILVSEFSHP RELASFIRRL DYDDGLYETY VEWKLKGKIS NQRLLTALNE REWGVQDINQ DNYIDSFECM VCRRVWANSR LQEQGLPPKQ WKADVSHLHC PEPALFTFSS PASPALRGRS LRELWLPSFQ QSKKEAQALR WLVDRNQNFS SEEFWALVFK D
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Fut10 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin-free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product FUT10 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    FUT10 (Fucosyltransferase 10 (Alpha (1,3) Fucosyltransferase) (FUT10))
    Alternative Name
    Fut10 (FUT10 Products)
    Synonyms
    FUT10 Protein, fut10A Protein, zgc:195147 Protein, fucosyltransferase 10 Protein, fucosyltransferase 10 (alpha (1,3) fucosyltransferase) L homeolog Protein, FUT10 Protein, Fut10 Protein, fut10 Protein, fut10.L Protein
    Background
    Probable fucosyltransferase. {ECO:0000250}.
    Molecular Weight
    56.9 kDa Including tag.
    UniProt
    Q5F2L2
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