SNRNP70 Protein (AA 2-448) (His tag)

Catalog No. ABIN3139890

Product Details

Target: SNRNP70 (Small Nuclear Ribonucleoprotein 70kDa (U1) (SNRNP70))

Protein Type: Recombinant

Protein Characteristics: AA 2-448

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This SNRNP70 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: TQFLPPNLLA LFAPRDPIPY LPPLEKLPHE KHHNQPYCGI APYIREFEDP RDAPPPTRAE TREERMERKR REKIERRQQE VETELKMWDP HNDPNAQGDA FKTLFVARVN YDTTESKLRR EFEVYGPIKR IHMVYSKRSG KPRGYAFIEY EHERDMHSAY KHADGKKIDG RRVLVDVERG RTVKGWRPRR LGGGLGGTRR GGADVNIRHS GRDDTSRYDE RPGPSPLPHR DRDRDRERER RERSRERDKE RERRRSRSRD RRRRSRSRDK DERRRSRERS KDKDRDRKRR SSRSRERARR ERERKEELRG GGGGGGGGSG GGGGGDMAEP SEAGDGAPDD GPPGELGPEG PDGPEEKGRD RDRERRRSHR SERERRRDRD RDRDREHKRG ERGSERGRDE ARGGGGSGQD NGLEGLGSDG RDMYMEAEGG DGYMAPENGY LMEAAPE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Snrnp70 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SNRNP70 (Small Nuclear Ribonucleoprotein 70kDa (U1) (SNRNP70))

Alternative Name: Snrnp70 (SNRNP70 Products)

Synonyms: rnpu1z Protein, rpu1 Protein, snrp70 Protein, u170k Protein, u1ap Protein, u1rnp Protein, SNRP70 Protein, wu:fc20b04 Protein, zgc:136450 Protein, 2700022N21Rik Protein, 3200002N22Rik Protein, 70kDa Protein, AI325098 Protein, R74807 Protein, Rnulp70 Protein, Snrp70 Protein, Srnp70 Protein, U1-70 Protein, U1-70K Protein, RNPU1Z Protein, RPU1 Protein, Snp1 Protein, U170K Protein, U1AP Protein, U1RNP Protein, small nuclear ribonucleoprotein, U1 70kDa subunit Protein, small nuclear ribonucleoprotein U1 subunit 70 Protein, small nuclear ribonucleoprotein 70 (U1) Protein, small nuclear ribonucleoprotein, U1 70kDa subunit L homeolog Protein, snrnp70 Protein, SNRNP70 Protein, Snrnp70 Protein, snrnp70.L Protein

Background: Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRNP70 binds to the loop I region of U1-snRNA. The truncated isoforms cannot bind U1-snRNA (By similarity). {ECO:0000250}.

Molecular Weight: 52.8 kDa Including tag.

UniProt: Q62376