SLC39A9 Protein (AA 1-308) (Strep Tag)

Catalog No. ABIN3124593

Product Details

Target: SLC39A9 (Solute Carrier Family 39 (Zinc Transporter), Member 9 (SLC39A9))

Protein Type: Recombinant

Protein Characteristics: AA 1-308

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SLC39A9 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MDDFLSISLL SVAMLVGCYV AGIIPLAVNF SEERLKLVTV LGAGLLCGTA LAVIVPEGVH ALYEEVLEGK HHQTSEMKQN GIASDKAAEI SSVHEHEHSH DHTQLHAYIG VSLVLGFVFM LLVDQIGSSH VHSSDDPETA RPSSSKITTT LGLVVHAAAD GVALGAAAST SQTSVQLIVF VAIMLHKAPA AFGLVSFLMH AGLERNRIRK HLLVFALAAP AMSMLTYLGL SKSSKEALSE VNATGVAMLF SAGTFLYVAT VHVLPEVGGM GHSHKPDTTG GRGLSRLEVA ALVLGCLIPL ILSIGHQH
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SLC39A9 (Solute Carrier Family 39 (Zinc Transporter), Member 9 (SLC39A9))

Alternative Name: Slc39a9 (SLC39A9 Products)

Synonyms: ZIP-9 Protein, ZIP9 Protein, 2010002A02Rik Protein, 2610511I23Rik Protein, 4833420E20Rik Protein, AW209151 Protein, slc39a9 Protein, MGC83699 Protein, MGC89410 Protein, wu:fc56g12 Protein, zgc:101628 Protein, DKFZp469D105 Protein, solute carrier family 39 member 9 Protein, solute carrier family 39, member 9 Protein, solute carrier family 39 (zinc transporter), member 9 Protein, solute carrier family 39 member 9 S homeolog Protein, solute carrier family 39 member 9 L homeolog Protein, SLC39A9 Protein, Slc39a9 Protein, slc39a9.S Protein, slc39a9.L Protein, slc39a9 Protein

Background: Zinc transporter ZIP9 (Solute carrier family 39 member 9) (Zrt- and Irt-like protein 9) (ZIP-9),FUNCTION: Transports zinc ions across cell and organelle membranes into the cytoplasm and regulates intracellular zinc homeostasis. Participates in the zinc ions efflux out of the secretory compartments. Regulates intracellular zinc level, resulting in the enhancement of AKT1 and MAPK3/MAPK1 (Erk1/2) phosphorylation in response to the BCR activation (By similarity). Also functions as a membrane androgen receptor that mediates, through a G protein, the non-classical androgen signaling pathway, characterized by the activation of MAPK3/MAPK1 (Erk1/2) and transcription factors CREB1 or ATF1 (PubMed:26208885). This pathway contributes to CLDN1 and CLDN5 expression and tight junction formation between adjacent Sertoli cells (By similarity). Mediates androgen-induced vascular endothelial cell proliferation through activation of an inhibitory G protein leading to the AKT1 and MAPK3/MAPK1 (Erk1/2) activation which in turn modulate inhibition (phosphorylation) of GSK3B and CCND1 transcription. Moreover, has dual functions as a membrane-bound androgen receptor and as an androgen-dependent zinc transporter both of which are mediated through an inhibitory G protein (Gi) that mediates both MAP kinase and zinc signaling leading to the androgen-dependent apoptotic process (By similarity). {ECO:0000250|UniProtKB:Q3KR82, ECO:0000250|UniProtKB:Q9NUM3, ECO:0000269|PubMed:26208885}.

Molecular Weight: 32.4 kDa

UniProt: Q8BFU1