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TOM70 Protein (AA 2-611) (rho-1D4 tag)

Crystallography grade TOMM70A Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Catalog No. ABIN3128750
  • Target See all TOM70 (TOMM70A) Proteins
    TOM70 (TOMM70A) (Translocase of Outer Mitochondrial Membrane 70 (TOMM70A))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 2-611
    Origin
    • 4
    • 1
    • 1
    Mouse
    Source
    • 3
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This TOM70 protein is labelled with rho-1D4 tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequence
    AASKPIEAAM AAAAAPGSGN GVGGGGGTAG PGSGAGTLPR WHVALAIGAP LLLGAGAMYL WSRRRRRREA GGRGDASGLK RNSERKTPEG RASPALGSGH HDGSGDSLEM SSLDRAQAAK NKGNKYFKAG KYEQAIQCYT EAISLCPTEK NVDLSTFYQN RAAAFEQLQK WKEVAQDCTK AVELNPKYVK ALFRRAKAHE KLDNKKECLE DVTAVCILEG FQNEQSMLLA DKVLKLLGKE NAKEKYKNRE PLMPSPQFIK SYFSSFTDDI ISQPMLKGEK SDEDKDKEGE ALEVKENSGY LKAKQYMEEE NYDKIISECS KEIDAQGKYM AEALLLRATF YLLIGSANAA KPDLDKVISL KEANVKLRAN ALIKRGTMCM QQQQPMLSTQ DFNMAAEIDP MNSDVYHHRG QLKILLDLVE EAVADFDACI RLRPKFALAQ AQKCFALYRQ AYTANNSSQV QAAMKGFEEI IKKFPRCAEG YALYAQALTD QQQFGKADEM YDKCIDLEPD NATTYVHKGL LQLQWKQDLD KGLELISKAI EIDNKCDFAY ETMGTIEVQR GNMEKAIDMF NKAINLAKSE MEMAHLYSLC DAAHAQTEVA KKYGLKPPTL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Tomm70 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin-free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product TOMM70A Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    TOM70 (TOMM70A) (Translocase of Outer Mitochondrial Membrane 70 (TOMM70A))
    Alternative Name
    Tomm70 (TOMM70A Products)
    Synonyms
    MGC68780 Protein, TOMM70A Protein, Tom70 Protein, KIAA0719 Protein, MGC73188 Protein, zgc:73188 Protein, wu:fj58b04 Protein, CG6756 Protein, Dmel\\CG6756 Protein, dtom70 Protein, GB13743 Protein, TOM70 Protein, 2610044B22Rik Protein, D16Ium22 Protein, D16Ium22e Protein, D16Wsu109e Protein, mKIAA0719 Protein, to translocase of outer mitochondrial membrane 70 S homeolog Protein, translocase of outer mitochondrial membrane 70 Protein, translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae) Protein, Translocase of outer membrane 70 Protein, mitochondrial import receptor subunit TOM70 Protein, translocase of outer mitochondrial membrane 70 homolog A (yeast) Protein, tomm70.S Protein, TOMM70 Protein, tomm70a Protein, tomm70 Protein, Tom70 Protein, LOC726081 Protein, Tomm70 Protein, Tomm70a Protein
    Background
    Receptor that accelerates the import of all mitochondrial precursor proteins. {ECO:0000250}.
    Molecular Weight
    68.6 kDa Including tag.
    UniProt
    Q9CZW5
    Pathways
    SARS-CoV-2 Protein Interactome
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