GDA Protein (AA 1-454) (His tag)
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- Target See all GDA Proteins
- GDA (Guanine Deaminase (GDA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-454
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Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This GDA protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- Sequence
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MCAARTPPLA LVFRGTFVHS TWTCPMEVLR DHLLGVSDSG KIVFLEESSQ QEKLAKEWCF KPCEIRELSH HEFFMPGLVD THIHAPQYAF AGSNVDLPLL EWLNKYTFPT EQRFRSTDVA EEVYTRVVRR TLKNGTTTAC YFGTIHTDSS LILAEITDKF GQRAFVGKVC MDLNDTVPEY KETTEESVKE TERFVSEMLQ KNYPRVKPIV TPRFTLSCTE TLMSELGNIA KTHDLYIQSH ISENREEIEA VKSLYPSYKN YTDVYDKNNL LTNKTVMAHG CYLSEEELNI FSERGASIAH CPNSNLSLSS GLLNVLEVLK HKVKIGLGTD VAGGYSYSML DAIRRAVMVS NVLLINKVNE KNLTLKEVFR LATLGGSQAL GLDSEIGNFE VGKEFDALLI NPRASDSPID LFYGDFVGDI SEAVIQKFLY LGDDRNIEEV YVGGKQVVPF SSSV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Gda Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product GDA Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- GDA (Guanine Deaminase (GDA))
- Alternative Name
- Gda (GDA Products)
- Synonyms
- CYPIN Protein, GUANASE Protein, NEDASIN Protein, AU015411 Protein, AW047581 Protein, Cypin Protein, MGC81835 Protein, zgc:112282 Protein, AGR_C_4212 Protein, DDBDRAFT_0169373 Protein, DDBDRAFT_0230211 Protein, DDB_0169373 Protein, DDB_0230211 Protein, guanine deaminase Protein, guanine deaminase L homeolog Protein, short-chain dehydrogenase/reductase sdr Protein, GDA Protein, Gda Protein, gda.L Protein, gda Protein, SPO2956 Protein, Gdia_3192 Protein, Mchl_2046 Protein, Mnod_4655 Protein, guaD Protein, Dd586_1771 Protein, Kvar_2512 Protein, Mrub_1462 Protein, BC1002_2263 Protein, Cseg_1079 Protein, Mesil_1795 Protein, Trad_2850 Protein, Mesop_0346 Protein, gDA Protein, ML5_2802 Protein
- Background
- Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.
- Molecular Weight
- 52.0 kDa Including tag.
- UniProt
- Q9R111
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