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ADAM17 Protein (AA 693-827) (His tag)

Crystallography grade ADAM17 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Catalog No. ABIN3146691
  • Target See all ADAM17 Proteins
    ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 693-827
    Origin
    • 7
    • 5
    • 1
    • 1
    Mouse
    Source
    • 6
    • 3
    • 3
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This ADAM17 protein is labelled with His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequence
    CVDKKLDKQY ESLSLFHHSN IEMLSSMDSA SVRIIKPFPA PQTPGRLQAL QPAAMMPPVP AAPKLDHQRM DTIQEDPSTD SHADDDGFEK DPFPNSSTAA KSFEDLTDHP VTRSEKAASF KLQRQSRVDS KETEC
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Adam17 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product ADAM17 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))
    Alternative Name
    Adam17 (ADAM17 Products)
    Synonyms
    ADAM18 Protein, CD156B Protein, CSVP Protein, NISBD Protein, TACE Protein, CD156b Protein, Tace Protein, adam17 Protein, si:dkey-81b15.2 Protein, adam18 Protein, cd156b Protein, csvp Protein, tace Protein, fc20b01 Protein, wu:fc20b01 Protein, zgc:63886 Protein, ADAM metallopeptidase domain 17 Protein, a disintegrin and metallopeptidase domain 17 Protein, ADAM metallopeptidase domain 17b Protein, ADAM metallopeptidase domain 17 L homeolog Protein, ADAM metallopeptidase domain 17a Protein, ADAM17 Protein, Adam17 Protein, adam17b Protein, adam17.L Protein, adam17a Protein
    Background
    Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Plays a role in the proteolytic processing of ACE2 (By similarity). Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called notch extracellular truncation (NEXT). {ECO:0000250, ECO:0000269|PubMed:10799547, ECO:0000269|PubMed:10882063, ECO:0000269|PubMed:11108241}.
    Molecular Weight
    16.0 kDa Including tag.
    UniProt
    Q9Z0F8
    Pathways
    Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus
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