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MAMDC4 Protein (AA 22-1228) (rho-1D4 tag)

Crystallography grade MAMDC4 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3130761
$39,912.43
Plus shipping costs $45.00 and $20.00 dry ice
Shipping to: United States
Delivery in 70 to 75 Business Days
  • Target See all MAMDC4 products
    MAMDC4 (MAM Domain Containing 4 (MAMDC4))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 22-1228
    Origin
    • 3
    • 1
    Mouse
    Source
    • 3
    • 1
    Insect Cells
    Purification tag / Conjugate
    This MAMDC4 protein is labelled with rho-1D4 tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    KTWLPNHCRS PIKAVCNFVC DCGDCSDETQ CGFHGASTIP STSFTCNFEQ DSCGWQDIST SGYRWLRDRA GAVLHGPGPH SDHTHGTDLG WYMAVGTHSG KEPSTATLRS PVMREAAPTC ELRLWYHIAS RDVAELRLDL THGVETLTLW QTSGPWGPGW QELAVNTGRI QGDFKVTFSA TRNATHRGAV ALDDVEFRDC GLPIPQARCP LGHHHCQNKA CVEPHQLCDG EDNCGDRSDE DPLICSHHMA TDFETGLGPW NQLEGWTRNH SAGSMVSPAW PHRDHSRNSA YGFFLISVAK PGTTAVLYSP EFQGSVSNNC SFTFYYYLHG SEASHFQLFL QAQGLNTPQV PVLLRSRHGE LGTAWVRDRV DIQSAHPFRI LLAGETGPGG VVGLDDLIMS SHCMLVPAMS TLQSSLSGPV PLALYPQTSI KLPQQTCEPG HLSCGDLCVP PEQLCDFQKH CAEGEDEHKC GTTDFESASA GGWEDISVGK LQWQWVEAQE KSKPAGDANR DAPGHFLSLQ KAWGQLRSEA RALTPALGPS GPHCELHMAY YFQSHPQGFL ALVVVENGFR ELLWQAPGGG SGSWTEEKII LGARRRPFQL EFVSLVDLDG PGQQGAGVDN VTLRDCNPMV TTESDQELSC NFERDSCSWH TGHLTDAHWH RIKSHGSQLD HTTGQGFFMF LDPTDPPARG QGALLLTRPQ VPVVPKECLS FWYRLYGPQI GTLCLAMRRE REEDILLWSR SGTHGNRWHQ AWVTLHHQPE ASTKYQLLFE GLRNGYHGTM ALDDIAVRPG PCWAPKSCSF EDSDCGFSPG GWGLWTHQSN ASGLASWGPW IDHTTGTAQG HYMVVDTSPN VLPKGHVAAL TSEEHQPLSQ PACLTFWYHM SVPNPGTLRV HVEESTRRQE LSISAHGRSA WRLGSVNVQA EQAWKVVFEA VAAGVEYSYM ALDDISLQDG PCPQPGSCDF ETGLCGWSHL PWPSLGGYSW DWSSGATPSR YPQPSVDHTL GTEAGHFAFF ETSVLGPGGQ AAWLRSEPLP ATTVSCLRFW YYMGFPEHFY KGELRVLLSS ARGQLAVWYQ GGHLRDQWLQ VQIELSNSEE FQIVFEATLG GQPALGPIAI DDVQYLAGQQ CKQPSPSQGE VAAPVSVPVA VGGALLFFMF LVLMGLGGWH WLQKQHCPGQ RSTDAAASGF ANILFNADHV TLPESITSNP QSPPDLA
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Mamdc4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin-free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    MAMDC4 (MAM Domain Containing 4 (MAMDC4))
    Alternative Name
    Mamdc4 (MAMDC4 Products)
    Synonyms
    AEGP Protein, Gm995 Protein, Aegp Protein, MAM domain containing 4 Protein, MAMDC4 Protein, Mamdc4 Protein
    Background
    Probably involved in the sorting and selective transport of receptors and ligands across polarized epithelia. {ECO:0000250|UniProtKB:Q63191}.
    Molecular Weight
    133.7 kDa Including tag.
    UniProt
    A2AJA7
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