SLC6A18 Protein (AA 1-615) (Strep Tag)

Catalog No. ABIN3132157

Product Details

Target: SLC6A18 (Solute Carrier Family 6, Member 18 (SLC6A18))

Protein Type: Recombinant

Protein Characteristics: AA 1-615

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SLC6A18 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MAQASGMDPL VDIEDERPKW DNKLQYLLSC IGFAVGLGNI WRFPYLCQTH GGGAFLIPYF IALVFEGIPL FYIELAIGQR LRRGSIGVWK TISPYLGGVG LGCFSVSFLV SLYYNTVLLW VLWFFLNSFQ HPLPWSTCPL DLNRTGFVQE CQSSGTVSYF WYRQTLNITS DISNTGTIQW KLFLCLVACW STVYLCVIRG IESTGKVIYF TALFPYLVLT IFLIRGLTLP GATEGLIYLF TPNMKTLQNP RVWLDAATQI FFSLSLAFGG HIAFASYNPP RNNCEKDAVI IALVNSMTSL YASIAIFSVM GFKASNDYGR CLDRNILSLI NEFDLPELSI SRDEYPSVLM YLNATQTARV AQLPLKTCHL EDFLDKSASG PGLAFIVFTE AVLHMPGASV WSVLFFGMLF TLGLSSMFGN MEGVITPLLD MGILPKGIPK EVMTGVICFA CFLSAICFTL QSGGYWLEIF DSFAASLNLI IFAFMEVVGV IHIYGMKRFC DDIEWMTGRR PGLYWQVTWR VVSPMLLFGI FLSYIVLLIQ TPPSYKAWNP QYEHFPSREE KFYPGWVQVT CVLLSFLPSL WVPGVALAQL LSQYKQRWKA THLESGLKLQ ESRGC
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SLC6A18 (Solute Carrier Family 6, Member 18 (SLC6A18))

Alternative Name: Slc6a18 (SLC6A18 Products)

Synonyms: SLC6A18 Protein, zgc:172267 Protein, B0AT3 Protein, D630001K16Rik Protein, Xt2 Protein, Xtrp2 Protein, Rosit Protein, solute carrier family 6 member 18 Protein, solute carrier family 6 (neutral amino acid transporter), member 18 Protein, solute carrier family 6 (neurotransmitter transporter), member 18 Protein, solute carrier family 6, member 18 Protein, SLC6A18 Protein, slc6a18 Protein, Slc6a18 Protein

Background: Sodium-dependent neutral amino acid transporter B(0)AT3 (Sodium- and chloride-dependent transporter XTRP2) (Solute carrier family 6 member 18) (System B(0) neutral amino acid transporter AT3),FUNCTION: Symporter that transports one amino acid molecule together with two sodium and one chloride ions in kidneys and plays a role in the neutral amino acids reabsorption (PubMed:19478081, PubMed:26240152, PubMed:20377526). Preferentially transports neutral amino acids such as L-glycine and L-alanine but also other neutral amino acids (PubMed:19478081, PubMed:26240152, PubMed:20377526). Required CLTRN for cell surface expression and for its amino acid transporter activity (PubMed:26240152, PubMed:20377526). The transport mechanism is pH -independent (PubMed:19478081). {ECO:0000269|PubMed:19478081, ECO:0000269|PubMed:20377526, ECO:0000269|PubMed:26240152}.

Molecular Weight: 69.2 kDa

UniProt: O88576