c-MYC Protein (AA 1-439) (His tag)

Catalog No. ABIN3148541

Product Details

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Protein Type: Recombinant

Protein Characteristics: AA 1-439

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This c-MYC protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: MPLNVNFTNR NYDLDYDSVQ PYFICDEEEN FYHQQQQSEL QPPAPSEDIW KKFELLPTPP LSPSRRSGLC SPSYVAVATS FSPREDDDGG GGNFSTADQL EMMTELLGGD MVNQSFICDP DDETFIKNII IQDCMWSGFS AAAKLVSEKL ASYQAARKDS TSLSPARGHS VCSTSSLYLQ DLTAAASECI DPSVVFPYPL NDSSSPKSCT SSDSTAFSPS SDSLLSSESS PRASPEPLVL HEETPPTTSS DSEEEQEDEE EIDVVSVEKR QTPAKRSESG SSPSRGHSKP PHSPLVLKRC HVSTHQHNYA APPSTRKDYP AAKRAKLDSG RVLKQISNNR KCSSPRSSDT EENDKRRTHN VLERQRRNEL KRSFFALRDQ IPELENNEKA PKVVILKKAT AYILSIQADE HKLTSEKDLL RKRREQLKHK LEQLRNSGA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Myc Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Alternative Name: Myc (MYC Products)

Synonyms: MRTL Protein, MYCC Protein, bHLHe39 Protein, c-Myc Protein, CMYC Protein, c-myc Protein, C-MYC Protein, LOC100136746 Protein, ARC Protein, B430311C09Rik Protein, MYC Protein, NOP Protein, Nop30 Protein, RNCMYC Protein, mMyc Protein, AU016757 Protein, Myc2 Protein, Niard Protein, Nird Protein, cmyc Protein, zc-myc Protein, c-myc II Protein, myc-B Protein, myc2 Protein, myc1 Protein, CMyc Protein, zgc:55680 Protein, CG10798 Protein, D-Myc Protein, DM Protein, DMYc Protein, Dm Protein, Dmel\CG10798 Protein, Dmyc Protein, EG:BACN5I9.1 Protein, anon-WO03040301.171 Protein, bHLHe57 Protein, c-MYC Protein, d-myc Protein, dMYC Protein, dMyc Protein, dMyc1 Protein, da Protein, dm Protein, dm/dMyc Protein, dm/myc Protein, dmyc Protein, dmyc1 Protein, l(1)G0139 Protein, l(1)G0354 Protein, l(1)G0359 Protein, myc Protein, MYC proto-oncogene, bHLH transcription factor Protein, v-myc myelocytomatosis viral oncogene homolog Protein, nucleolar protein 3 (apoptosis repressor with CARD domain) Protein, myelocytomatosis oncogene Protein, MYC proto-oncogene, bHLH transcription factor a Protein, MYC proto-oncogene, bHLH transcription factor L homeolog Protein, MYC proto-oncogene, bHLH transcription factor S homeolog Protein, v-myc myelocytomatosis viral oncogene homolog (avian) Protein, MYC proto-oncogene, bHLH transcription factor b Protein, CG10798 gene product from transcript CG10798-RB Protein, MYC Protein, LOC100136746 Protein, Nol3 Protein, Myc Protein, myca Protein, myc.L Protein, myc.S Protein, mycb Protein

Background: Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes.

Molecular Weight: 49.9 kDa Including tag.

UniProt: P01108

Pathways: p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect