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Akr1b7 Protein (AA 2-316) (His tag)

Crystallography grade AKR1B7 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3149457
  • Target
    Akr1b7 (AKR1B7) (Aldo-Keto Reductase Family 1, Member B7 (AKR1B7))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 2-316
    Origin
    Mouse
    Source
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This Akr1b7 protein is labelled with His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    ATFVELSTKA KMPLVGLGTW KSSPGQVKEA VKAAIDAGYR HIDCAYVYHN ENEVGEAIQE KIKENAVKRE DLFIVSKLWA TFFEKSLVKK AFQNTLSDLK LDYLDLYLVH WPQGFQAGNA LLPKDNKGKV LLSKSTFLDA WEAMEELVDQ GLVKALGISN FNHFQIERLL NKPGLKHKPV TNQIESHPYL TQEKLIQYCQ SKGIAVTAYS PLGSPDRPYA KPEDPVVMEI PKIKEIAAKH KKTVAQVLIR FHVQRNVVVI PKSVTPSRIQ ENLQVFDFQL SEEDMAAILS FNRNWRACDL LDARTEEDYP FHEEY
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Akr1b7 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    Akr1b7 (AKR1B7) (Aldo-Keto Reductase Family 1, Member B7 (AKR1B7))
    Alternative Name
    Akr1b7
    Synonyms
    AR Protein, Avdp Protein, MVDP Protein, aldo-keto reductase family 1, member B7 Protein, Akr1b7 Protein
    Background
    Reduces a broad range of aliphatic and aromatic aldehydes to the corresponding alcohols. May play a role in the metabolism of xenobiotic aromatic aldehydes (By similarity). The role of MVDP in sperm maturation and storage may be related to its potential capacity to produce fructose, or MVDP may play an osmoregulatory role by producing sorbitol. {ECO:0000250}.
    Molecular Weight
    36.8 kDa Including tag.
    UniProt
    P21300
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