SUPT4H Protein (AA 2-117) (His tag)

Catalog No. ABIN3151225

Product Details

Target: SUPT4H (SUPT4H1) (Suppressor of Ty 4 Homolog 1 (SUPT4H1))

Protein Type: Recombinant

Protein Characteristics: AA 2-117

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This SUPT4H protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: ALETVPKDLR HLRACLLCSL VKTIDQFEYD GCDNCDAYLQ MKGNREMVYD CTSSSFDGII AMMSPEDSWV SKWQRVSNFK PGVYAVSVTG RLPQGIVREL KSRGVAYKSR DTAIKT
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Supt4h1a Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SUPT4H (SUPT4H1) (Suppressor of Ty 4 Homolog 1 (SUPT4H1))

Alternative Name: Supt4h1a (SUPT4H1 Products)

Synonyms: SPT4 Protein, SPT4H Protein, SUPT4H Protein, Supt4h2 Protein, AL022777 Protein, Supt4h Protein, Supt4h1 Protein, Supt4h1a Protein, 142904_at Protein, CG12372 Protein, DSIF Protein, Dmel\\CG12372 Protein, Dspt4 Protein, Spt4 Protein, dDSIF Protein, dspt4 Protein, l(2)k05316 Protein, DSIF small subunit Protein, spt4 Protein, zgc:92824 Protein, SPT4 homolog, DSIF elongation factor subunit Protein, suppressor of Ty 4A Protein, Transcription elongation factor SPT4 Protein, CG12372 gene product from transcript CG12372-RA Protein, SPT4 homolog, DSIF elongation factor subunit L homeolog Protein, transcription elongation factor Protein, transcription elongation factor complex subunit Spt4 Protein, SUPT4H1 Protein, Supt4h1 Protein, Supt4a Protein, spt-4 Protein, spt4 Protein, supt4h1.L Protein, CAALFM_C504630WA Protein, supt4h1 Protein

Background: Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites (By similarity). {ECO:0000250}.

Molecular Weight: 14.0 kDa Including tag.

UniProt: P63271