CD13 Protein (AA 2-966) (rho-1D4 tag)

Catalog No. ABIN3134706

Product Details

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Protein Type: Recombinant

Protein Characteristics: AA 2-966

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This CD13 protein is labelled with rho-1D4 tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: AKGFYISKTL GILGILLGVA AVCTIIALSV VYAQEKNRNA ENSATAPTLP GSTSATTATT TPAVDESKPW NQYRLPKTLI PDSYRVILRP YLTPNNQGLY IFQGNSTVRF TCNQTTDVII IHSKKLNYTL KGNHRVVLRT LDGTPAPNID KTELVERTEY LVVHLQGSLV EGRQYEMDSQ FQGELADDLA GFYRSEYMEG DVKKVVATTQ MQAADARKSF PCFDEPAMKA MFNITLIYPN NLIALSNMLP KESKPYPEDP SCTMTEFHST PKMSTYLLAY IVSEFKNISS VSANGVQIGI WARPSAIDEG QGDYALNVTG PILNFFAQHY NTSYPLPKSD QIALPDFNAG AMENWGLVTY RESSLVFDSQ SSSISNKERV VTVIAHELAH QWFGNLVTVA WWNDLWLNEG FASYVEYLGA DYAEPTWNLK DLMVLNDVYR VMAVDALASS HPLSSPADEI KTPDQIMELF DSITYSKGAS VIRMLSSFLT EDLFKKGLSS YLHTYQYSNT VYLDLWEHLQ KAVNQQTAVQ PPATVRTIMD RWILQMGFPV ITVNTNTGEI SQKHFLLDSK SNVTRPSEFN YIWIAPIPFL KSGQEDHYWL DVEKNQSAKF QTSSNEWILL NINVTGYYLV NYDENNWKKL QNQLQTDLSV IPVINRAQII HDSFNLASAK MIPITLALDN TLFLVKEAEY MPWQAALSSL NYFTLMFDRS EVYGPMKRYL KKQVTPLFFY FQNRTNNWVN RPPTLMEQYN EINAISTACS SGLKECRDLV VELYSQWMKN PNNNTIHPNL RSTVYCNAIA FGGEEEWNFA WEQFRNATLV NEADKLRSAL ACSKDVWILN RYLSYTLNPD YIRKQDTTST IISIASNVAG HPLVWDFVRS NWKKLFENYG GGSFSFANLI QGVTRRFSSE FELQQLEQFK ADNSATGFGT GTRALEQALE KTRANIDWVK ENKDAVFKWF TENSS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Anpep Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin-free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Alternative Name: Anpep (ANPEP Products)

Synonyms: APN Protein, CD13 Protein, GP150 Protein, LAP1 Protein, P150 Protein, PEPN Protein, A2AP Protein, AAP Protein, ALPHA-2-PI Protein, API Protein, PLI Protein, AAT1 Protein, ALT1 Protein, GPT1 Protein, Gpt1 Protein, Apn Protein, pepN Protein, SC8E4A.13 Protein, 1300007J06Rik Protein, 2310022B03Rik Protein, ALT Protein, Gpt-1 Protein, AP-M Protein, AP-N Protein, Cd13 Protein, Apm Protein, KZP Protein, Lap1 Protein, rAPN Protein, APDE Protein, p150 Protein, anpep Protein, fb64c05 Protein, wu:fb64c05 Protein, zgc:136771 Protein, alanyl aminopeptidase, membrane Protein, serpin family F member 2 Protein, glutamic--pyruvic transaminase Protein, aminopeptidase N Protein, aminopeptidase Protein, aminopeptidase n Protein, glutamic pyruvic transaminase, soluble Protein, alanyl (membrane) aminopeptidase Protein, membrane alanyl aminopeptidase Protein, alanyl (membrane) aminopeptidase b Protein, alanyl aminopeptidase, membrane L homeolog Protein, ANPEP Protein, SERPINF2 Protein, GPT Protein, Gpt Protein, Apn2 Protein, Apn1 Protein, SCO2643 Protein, pepN Protein, Shewana3_0897 Protein, Alvin_1024 Protein, Ndas_3413 Protein, Saut_1544 Protein, Fbal_1877 Protein, ML5_3424 Protein, Despr_1988 Protein, Mesop_5365 Protein, Anpep Protein, LOC101744250 Protein, anpepb Protein, anpep.L Protein

Background: Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May have a role in angiogenesis (By similarity). Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells. {ECO:0000250, ECO:0000269|PubMed:8103749}.

Molecular Weight: 110.7 kDa Including tag.

UniProt: P97449

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones