DDR1 Protein (AA 20-911) (rho-1D4 tag)

Catalog No. ABIN3134807

Product Details

Target: DDR1 (Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))

Protein Type: Recombinant

Protein Characteristics: AA 20-911

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This DDR1 protein is labelled with rho-1D4 tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: DADMKGHFDP AKCRYALGMQ DRTIPDSDIS VSSSWSDSTA ARHSRLESSD GDGAWCPAGP VFPKEEEYLQ VDLRRLHLVA LVGTQGRHAG GLGKEFSRSY RLRYSRDGRR WMDWKDRWGQ EVISGNEDPG GVVLKDLGPP MVARLVRFYP RADRVMSVCL RVELYGCLWR DGLLSYTAPV GQTMQLSEVM VHLNDSTYDG YTAGGLQYGG LGQLADGVVG LDDFRQSQEL RVWPGYDYVG WSNQSFPTGY VEMEFEFDRL RTFQTMQVHC NNMHTLGARL PGGVECRFKR GPAMAWEGEP VRHALGGSLG DPRARAISVP LGGHVGRFLQ CRFLFAGPWL LFSEISFISD VVNDSSDTFP PAPWWPPGPP PTNFSSLELE PRGQQPVAKA EGSPTAILIG CLVAIILLLL LIIALMLWRL HWRRLLSKAE RRVLEEELTV HLSVPGDTIL INNRPGPREP PPYQEPRPRG TPPHSAPCVP NGSALLLSNP AYRLLLATYA RPPRGPGPPT PAWAKPTNTQ ACSGDYMEPE KPGAPLLPPP PQNSVPHYAE ADIVTLQGVT GGNTYAVPAL PPGAVGDGPP RVDFPRSRLR FKEKLGEGQF GEVHLCEVED PQDLVSSDFP ISVHKGHPLL VAVKILRPDA TKNARNDFLK EVKIMSRLKD PNIIRLLGVC VQDDPLCMIT DYMENGDLNQ FLSARQLENK ATQGLSGDTE SDQGPTISYP MLLHVGAQIA SGMRYLATLN FVHRDLATRN CLVGENFTIK IADFGMSRNL YAGDYYRVQG RAVLPIRWMA WECILMGKFT TASDVWAFGV TLWEVLMLCR SQPFGQLTDE QVIENAGEFF RDQGRQVYLS RPPACPQTLY ELMLRCWSRE PEQRPPFAQL HRFLADDALN TV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Ddr1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin-free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: DDR1 (Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))

Alternative Name: Ddr1 (DDR1 Products)

Synonyms: CG11573 Protein, CG13984 Protein, CG13985 Protein, CG33531 Protein, CG9488 Protein, CG9490 Protein, CT33540 Protein, DDR Protein, Dmel\\CG33531 Protein, Dmel_CG13984 Protein, 6030432F18 Protein, AI323681 Protein, CD167a Protein, Cak Protein, Nep Protein, PTK3A Protein, CAK Protein, CD167 Protein, EDDR1 Protein, HGK2 Protein, MCK10 Protein, NEP Protein, NTRK4 Protein, PTK3 Protein, RTK6 Protein, TRKE Protein, Drd1 Protein, PTK3D Protein, Discoidin domain receptor Protein, discoidin domain receptor family, member 1 Protein, discoidin domain receptor tyrosine kinase 1 Protein, Ddr Protein, Ddr1 Protein, DDR1 Protein

Background: Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing, but also tumor cell invasion. Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Phosphorylates PTPN11 (By similarity). Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing. {ECO:0000250, ECO:0000269|PubMed:12065315}.

Molecular Weight: 100.4 kDa Including tag.

UniProt: Q03146

Pathways: RTK Signaling, Smooth Muscle Cell Migration