FBXW10 Protein (AA 1-1030) (Strep Tag)

Catalog No. ABIN3152117

Product Details

Target: FBXW10 (F-Box and WD Repeat Domain Containing 10 (FBXW10))

Protein Type: Recombinant

Protein Characteristics: AA 1-1030

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This FBXW10 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MENREPKLKQ APYFRCEKGP NWVPVCQKCE ACVLAWKIFA TKEWFRRVND ISQRRFLVSI LGQLNSLYLL QYFQNILETT QGKDFIYNRS RIKLSRKGGK EEEVVKSSLN QMLDKTVERK MKEILYWFGN STHRTKANYT LLLLQMCDSN LLLTAANVIR VLFMKEWNSI SGLHDDTPDV MFFPEKKYSG TQDTSYVSWA ARPKPVSFPM SKHLGNKLGT ENVDRETTEG KGESSLQCIH EMNRQIFGKG GMSRLGDDPC NLLLSLDHVQ LLSSGYSKYR DFIRDLPLHL SKYILRMLDK HSLNRCIFVS QHWATLAQQV KVDQSMHSFI QNQISLLQVT KEEKQAYTGS YTRGIDPNYA NKVSIPVPKI VDDGKRSRSK NQKWKLRTKT DYNLWNAYQN QETQLVQMEE RNVFCGTYNI RVLSDTFDQN RIIHYNGGDL MAISSNRKIH LLDIMQTKEL PIEFRGHAGS VRALFLSEED NILLSGSYDL SIRYWDVKTG ACVRIFYGHQ GTITCLDVYK NRLVSGAKDG QVKEWDIETG KCLKTFKHKD PILAAKISET YIVSSCERGI VKVWHVVTAQ LQKTLTGHEG AVKCLFFNEW HLVSGGADGL VMAWSMVGKY ERCLMAFKHP KEVLQVSLLY LRVISACGDG KIRIYNFLNG NCLKVIKVDA RGDPVLSFFY QGNRMVAHTD SNILVFQFEN VKWQYSSDKN KVKKSKDKEE EREETSLGDE HSRSTIQGHS LKDSVSSKQE FSKSRVHLKQ TKNLSSDDME TPVGEVSHPL QKLWKVPMTP DRFLLTISAL QQAHNSEEFA YPHRPRPQVI DAWGPSIPYP RKVLSLKGKS VQHAVDQLRS SNLPTGVRQT NIPLEIQKLQ PNLKKSLHSP RVQATVPQPS LIRPKVSDSL RGDEHLTSSI DGTMRRAGPL TSMQVIKPNR MLAPRGGTAT LSPKKERPRF YTTLDPLRMN TGFMLMTVKE EKEFAEAKMK EYEASVSTKE VDPGKASKAA WIRKIKGLPI DNFMKEGKTA APELGQNVFI
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: FBXW10 (F-Box and WD Repeat Domain Containing 10 (FBXW10))

Alternative Name: Fbxw10 (FBXW10 Products)

Synonyms: Fbw10 Protein, HREP Protein, SM25H2 Protein, SM2SH2 Protein, CMT1A duplicated region transcript 1 Protein, F-box and WD repeat domain containing 10 Protein, tripartite motif containing 16 Protein, F-box and WD-40 domain protein 10 Protein, CDRT1 Protein, FBXW10 Protein, Fbxw10 Protein, Cdrt1 Protein, TRIM16 Protein

Background: F-box/WD repeat-containing protein 10 (F-box and WD-40 domain-containing protein 10),FUNCTION: Probable substrate-recognition component of a SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Overexpression is leading to degradation of CBX5 and CBX1 (By similarity). {ECO:0000250}.

Molecular Weight: 117.7 kDa

UniProt: Q5SUS0