SLC6A4 Protein (AA 1-630) (rho-1D4 tag)
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- Target See all SLC6A4 Proteins
- SLC6A4 (Solute Carrier Family 6 (Neurotransmitter Transporter, serotonin), Member 4 (SLC6A4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-630
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Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This SLC6A4 protein is labelled with rho-1D4 tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- Sequence
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METTPLNSQK VLSECKDKED CQENGVLQKG VPTPADKAGP GQISNGYSAV PSTSAGDEAP HSTPAATTTL VAEIHQGERE TWGKKMDFLL SVIGYAVDLG NIWRFPYICY QNGGGAFLLP YTIMAIFGGI PLFYMELALG QYHRNGCISI WKKICPIFKG IGYAICIIAF YIASYYNTII AWALYYLISS FTDQLPWTSC KNSWNTGNCT NYFAQDNITW TLHSTSPAEE FYLRHVLQIH QSKGLQDLGT ISWQLALCIM LIFTIIYFSI WKGVKTSGKV VWVTATFPYI VLSVLLVRGA TLPGAWRGVV FYLKPNWQKL LETGVWVDAA AQIFFSLGPG FGVLLAFASY NKFNNNCYQD ALVTSVVNCM TSFVSGFVIF TVLGYMAEMR NEDVSEVAKD AGPSLLFITY AEAIANMPAS TFFAIIFFLM LITLGLDSTF AGLEGVITAV LDEFPHIWAK RREWFVLIVV ITCILGSLLT LTSGGAYVVT LLEEYATGPA VLTVALIEAV VVSWFYGITQ FCSDVKEMLG FSPGWFWRIC WVAISPLFLL FIICSFLMSP PQLRLFQYNY PHWSIILGYC IGTSSVICIP IYIIYRLIST PGTLKERIIK SITPETPTEI PCGDIRMNAV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Slc6a4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin-free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product SLC6A4 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- SLC6A4 (Solute Carrier Family 6 (Neurotransmitter Transporter, serotonin), Member 4 (SLC6A4))
- Alternative Name
- Slc6a4 (SLC6A4 Products)
- Synonyms
- 5-HTT Protein, 5-HTTLPR Protein, 5HTT Protein, HTT Protein, OCD1 Protein, SERT Protein, SERT1 Protein, hSERT Protein, AI323329 Protein, Htt Protein, Sert Protein, CG4545 Protein, DmSERT Protein, DmSerT Protein, Dmel\\CG4545 Protein, dSERT Protein, dSERT1 Protein, dSerT Protein, dSert Protein, dsert Protein, SLC6A4 Protein, solute carrier family 6 member 4 Protein, solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 Protein, Serotonin transporter Protein, huntingtin Protein, solute carrier family 6 (neurotransmitter transporter), member 4b Protein, solute carrier family 6 (neurotransmitter transporter), member 4a Protein, SLC6A4 Protein, Slc6a4 Protein, SerT Protein, LOC100540532 Protein, slc6a4 Protein, slc6a4b Protein, slc6a4a Protein
- Background
- Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner. {ECO:0000269|PubMed:9037532}.
- Molecular Weight
- 71.2 kDa Including tag.
- UniProt
- Q60857
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